Caspase-8 Antibody

Caspase-8 Antibody

Catalog# : 3475

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death (reviewed in 3). Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex (4). The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation (reviewed in 5). FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis (6). This antibody will only detect isoform E of caspase-8.

Additional Names : Caspase-8 (E), FLICE, MACH ,Mch5


Description
Left: Western blot analysis of Caspase-8 in HT-29 cell lysate with Caspase-8 antibody (IN) at 1 µg/ml in (A) the presence or (B) the absence of blocking peptide.

Source : Caspase-8 antibody was raised against a 15 amino acid peptide from near the carboxy terminus human caspase-8 isoform E.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Caspase-8 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Virus Casp-8 / FLICE / MACH / Mch5 (E) Peptide (Cat. No. 3475P)

Application : Casp-8 antibody can be used for the detection of Caspase-8 by Western blot at 0.5 µg/ml.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3475P - Casp-8 Peptide

Long-Term Storage : Caspase-8 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.HT-29 Cell Lysate

Species Reactivity :H, M

GI Number : 15718704

Accession Number : NP_001219

Short Description : (E) an initiator caspase

References

1.Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2.Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3.Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
4.Muzio M, Chinnaiyan AM, Kischkel FC, et al. FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex. Cell 1996; 85:817-27.