CARMA1 Antibody

CARMA1 Antibody

Catalog# : 3189

CARMA proteins belong to the membrane-associated guan-ylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules (1). CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types (2-4). Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the I?K complex, thereby regulating activation of the NF-kappaB transcription factor (5). Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems (6).

Additional Names : CARMA1
Description
Left: Western blot analysis of CARMA1 expression in mouse thymus cell lysate with CARMA1 antibody at 0.5 (lane A), 1 (lane B), and 2 µg /ml (lane C), respectively.

Below: Immunohistochemistry of CARMA1 in mouse thymus with CARMA1 antibody at 10 µg/ml.

Other Product Images


Source : CARMA1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : CARMA1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human CARMA1 Peptide (Cat. No. 3189 P)

Application : CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 µg/ml.A band at approximately 125 kDa can be detected.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3189P - CARMA1 Peptide

Long-Term Storage : CARMA1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1409 - Mouse Thymus Tissue Lysate

Species Reactivity :H, M

GI Number : 172046231

Accession Number : Q9BXL7

Short Description : lymphocyte activating CARD-containing protein

References
1.Fanning AS and Anderson JM. Protein modules as organizers of membrane structure.Curr. Opin. Cell Biol. 1999; 11:432-9.
2.Gaide O, Martinon F, Michau O, et al. Carma1, 1 CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappa B activation. FEBS Lett. 2001; 496:121-7.
3.Bertin J, Wang L, Guo Y, et al. CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B. J. Biol. Chem. 2001; 276:11877-82.
4.McAllister-Lucas LM, Inohara N, Lucas PC, et al. Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10-mediated NF-kappaB induction. J. Biol. Chem. 2001; 276:30589-97.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis