Catalog# : 3465
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death (reviewed in 3). Caspase-7 is an executioner caspase that was identified based on its homology with caspases 1 and 3, as well as the C. elegans cell death protein CED-3 (4). Alternative splicing of Caspase-7 mRNA results in the production of 3 distinct isoforms (4). Caspase-7 activity can be directly inhibited by XIAP expression (5).
Additional Names : Caspase-7 (CT), ICELAP3 ,Mch3
DescriptionLeft: Western blot analysis of Caspase-7 in human skeletal muscle cell lysate with Caspase-7 antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Below: Immunohistochemical staining of human skeletal muscle using Caspase-7 antibody at 2 µg/ml.
Other Product Images
Source : Caspase-7 antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human Caspase-7.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Caspase-7 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human Casp-7 / ICELAP3 / Mch3 (C-Terminus) Peptide (Cat. No. 3465P)
Application : Casp-7 antibody can be used for the detection of Caspase-7 by Western blot at 0.5 µg/ml.Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3465P - Casp-7 Peptide
Long-Term Storage : Caspase-7 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1.Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Species Reactivity :H, M, R
GI Number : 1730092
Accession Number : P55210
Short Description : (CT) an executioner caspase
References
1.Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2.Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3.Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
4.Juan TSC, McNiece IK, Argento JM, et al. Identification and mapping of Casp7, a cysteine protease resembling CPP32b, Interleukin-1b converting enzyme, and CED-3. Genomics 1997; 40:86-93.
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