Caspase-4 Antibody

Caspase-4 Antibody

Catalog# : 3451

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18 (2,3). Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells (4). More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer’s disease (5).

Additional Names : Caspase-4 (NT), ICH-2, ICE rel II , Mih1
Description
Left: Western blot analysis of caspase-4 in Ramos cells with caspase-4 antibody at (A) 0.5 and (B) 1 µgg/ml.

Below: Immunohistochemical staining of mouse spleen using caspase-4 antibody at 2 µg/ml.

Other Product Images
Source : Caspase-4 antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-4.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Caspase-4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human Casp-4 / ICH-2 / ICE rel II / Mih1 (N-Terminus) Peptide (Cat. No. 3451P)

Application : Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 1 µg/ml.Depending on cell lines or tissues used, other cleavage products may be observed.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3451P - Casp-4 Peptide

Long-Term Storage : Caspase-4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1225 - Ramos Cell Lysate
2.Cat. No. 1406 - Mouse Spleen Tissue Lysate

Species Reactivity :H, M

GI Number : 886050

Accession Number : AAA86890

Short Description : (NT) a caspase induced by ER stress

References

1.Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2.Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science 1995; 267:2000-3.
3.Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
4.Kamens J, Paskind M, Hugunin M, et al. Identification and characterization of ICH-2, a novel member of the interleukin-1 beta-converting enzyme family of cysteine proteases. J. Biol. Chem. 1995; 270:15250-6.