Caspase-12 Antibody (Large)

Caspase-12 Antibody (Large)

Catalog# : 3195

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including ?-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues (1).

Additional Names : Caspase-12 (large), Casp-12 (lrg)
Description
Left: Western blot analysis of caspase-12 in human heart lysate with caspase-12 antibody (large) at 0.5 (lane A), 1 (lane B), and 2 µg/ml (lane C), respectively.

Below: Immunohistochemical staining of human heart tissue using caspase-12 antibody (large) at 2 µg/ml.

Source : Caspase-12 antibody was raised against a synthetic peptide corresponding to amino acids within the large cleavage product of murine caspase-12.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Caspase-12 antibody (Large) was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Mouse Caspase-12 (Large) Peptide (Cat. No. 3195P)

Application : Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.5 to 2 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3195P - Caspase-12 (Large) Peptide

Long-Term Storage : Caspase-12 antibody (Large) can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control 1. Cat. No. 1301 - Human Heart Tissue Lysate

Species Reactivity :H, M, R

GI Number : 2094806

Accession Number : CAA73532

Short Description : A new caspase

References 1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98-103. 2. Mehmet H. Caspases find a new place to hide. Nature 2000;403:29-30 3. Van de Craen M, Vandenabeele P, Declercq W, Van den Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W, Beyaert R, Fiers W. Characterization of seven murine caspase family members. FEBS Lett 1997;403:61-9 For more detail regarding Peptide please visit here : Custom Peptide Synthesis