Catalog# : 3463
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1? and IL-18 (3,4). Caspase-1 was initially identified as an IL-1beta-converting enzyme (5); later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 (6) whose overexpression can induce apoptosis in fibroblasts (7).
Additional Names : Caspase-1 (IN), ICE, IL-1beta converting enzyme
DescriptionLeft: Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Below: Immunohistochemistry of caspase-1 in human spleen tissue with caspase-1 antibody at 10 µg/ml.
Other Product Images
Source : Caspase-1 antibody was raised against a 15 amino acid peptide from near the middle of human Caspase-1.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Caspase-1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human Casp-1 / ICE / IL-1b converting enzyme (Intermediate Domain) Peptide (Cat. No. 3463P)
Application : Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 µg/ml.Depending on cell lines or tissues used, other cleavage products may be observed.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3463P - Casp-1 Peptides
Long-Term Storage : Caspase-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1.Cat. No. 1201 - HeLa Cell Lysate
2.Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H
GI Number : 15431328
Accession Number : NP_150634
Short Description : (IN) a caspase in the inflammatory subfamily
References
1.Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. CCell 2004; 117:561-74.
2.Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3.Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science 1995; 267:2000-3.
4.Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
For more detail regarding Peptide please visit here : Custom Peptide Synthesis
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