Caspase-9 Antibody

Caspase-9 Antibody

Catalog# : 2071

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1,2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1,2).

Additional Names : Caspase-9 (IN1), Mch6/ Apaf-3, Casp-9

Description
Left: Western blot analysis of caspase-9 in HeLa whole cell lysate with caspase-9 antibody at 1:1000 dilution.



Source : Caspase-9 antibody was raised against a peptide corresponding to amino acids 41 to 56 of human caspase-9 .

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Caspase-9 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human Caspase-9 (Intermediate Domain 1) Peptide (Cat. No. 2071P)

Application : Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution. A 46 kDa band should be detected. It has no cross response to other members in caspase family.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2071P - Caspase-9 Peptide

Long-Term Storage : Caspase-9 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity :H, M, R

GI Number : 28558771

Accession Number : P55211

Short Description : (IN1) A new caspase

References

1.Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4
2.Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J Biol Chem 1996;271:27099-106
3.Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
4.Pan G, O'Rourke K, Dixit VM. Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex. J Biol Chem 1998;273:5841-5 (RD1299)