Caspase-10 Antibody

Caspase-10 Antibody

Catalog# : 1128

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch41,2 and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis1. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.

Additional Names : Caspase-10 (CT), Casp-10, FLICE2
Description
Left: Western blot analysis of caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody at 1 µg/ml.


Source : Caspase-10 antibody was raised against a peptide corresponding to amino acids 505 to 521 of human FLICE2.

Purification : Antibody is DEAE purified

Clonality and Clone : This is a polyclonal antibody.

Host : Caspase-10 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human Caspase-10 (C-Terminus) Peptides (Cat. No. 1128P)

Application : Caspase-10 antibody can be used for detection of caspase-10 by Western blot at 1:500 to 1:1000 dilution. A 59 kDa band can be detected. Antibody only recognizes FLICE2 form of caspase-10.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 1128P - Caspase-10 Peptide

Long-Term Storage : Caspase-10 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1201 - HeLa Cell Lysate
2.Cat. No. 1205 - Jurkat Whole Cell Lysate
3.Cat. No. 1202 - A431 Cell Lysate
4.Cat. No. 1204 - K562 Cell Lysate

Species Reactivity :H

GI Number : 4731237

Accession Number : AAD28402

Short Description : (CT) A new caspase

References

1.Vincenz.C. and Dixit,V.M. Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem. 1997;272:6578-6583
2.Fernandes-Alnemri T, Armstrong RC, Krebs J, Srinivasula SM, Wang L Bullrich F, Fritz LC, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci. USA. 1996;93:7464-7469
3.Cohen GM. Caspases: the executioners of apoptosis. Biochem J 1997;326:1-16 (RD1299)

For more detail regarding Peptide please visit here : Custom Peptide Synthesis