Catalog# : 3471
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death (reviewed in 3). Caspase-6 is an executioner caspase that was idientifed based on its homology with human caspases 2 and 3 as well as the C. elegans cell death protein CED-3 (4). It possesses two isoforms, of which only the longer form possesses protease activity (4). Caspase-6 is highly expressed in adult brain and may play a role in several neuronal pathologies (5).
Additional Names : Caspase-6 (NT), Mch2
DescriptionLeft: Western blot analysis of caspase-6 in Jurkat cell lysate with caspase-6 antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Below: Immunocytochemistry of caspase-6 in Jurkat cells with caspase-6 antibody at 2 µg/ml.
Other Product Images
Source : Caspase-6 antibody was raised against a 15 amino acid peptide from near the amino-terminus of human Caspase-6.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Caspase-6 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Virus Casp-6 / Mch2 (N-Terminus) Peptide (Cat. No. 3471P)
Application : Caspase-6 antibody can be used for the detection of caspase-6 by Western blot at 0.5 µg/ml.Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3471P - Casp-6 Peptide
Long-Term Storage : Caspase-6 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1.Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity :H
GI Number : 14916483
Accession Number : NP_001217
Short Description : (NT) an executioner caspase
References
1.Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2.Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3.Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
4.Fernandes-Alnemri T, Litwack G, and Alnemri ES. Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family. Cancer Res. 1995; 55:2737-42.
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