Caspase-13 Antibody

Caspase-13 Antibody

Catalog# : 2245

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death is finally caused by members of the caspase family of proteases and caspase activated DNases. A novel member in the caspase family was recently identified and designated ERICE (for Evolutionarily Related Interleukin-1 b Converting Enzyme) and caspase-13?(1). Caspase-13 belongs to the ICE subfamily of caspases. Overexpression of caspase-13 induces apoptosis. Caspase-13 was activated by caspase-8, which is a key enzyme in death receptor induced apoptosis. Caspase-13 is expressed in a variety of human tissues and cell lines (1).

Additional Names : Caspase-13, Casp-13, ERICE
Description
Left: Western blot analysis of caspase-13 in human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 µg/ml.

Below: Immunohistochemistry of caspase-13 in mouse brain tissue with caspase-13 antibody at 10 µg/ml.

Other Product Images
Source : Caspase-13 antibody was raised against a peptide corresponding to amino acids 91 to 105 of human caspase-13.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : Caspase-13 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human Caspase-13 Peptide (Cat. No. 2245P)

Application : Caspase-13 antibody can be used for detection of caspase-13 by Western blot at 0.5 to 1 µg/ml.A 43 kDa band should be detected.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2245P - Caspase-13 Peptide

Long-Term Storage : Caspase-13 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1209 - HL60 Cell Lysate
2.Cat. No. 1403 - Mouse Brain Tissue Lysate
3.Cat. No. 1463 - Rat Brain Tissue Lysate

Species Reactivity :H, M, R

GI Number : 10720034

Accession Number : O75601

Short Description : Evolutionarily Related ICE

References

1.Humke EW, Ni J, Dixit VM. ERICE, a novel FLICE-activatable caspase. J Biol Chem 1998;273:15702-7 (WD0500)