cIAP Antibody

cIAP Antibody

Catalog# : 3325

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases (1 for review). The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases–3, –7, (2,3). The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-alpha by its receptor (2,4). Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP (5). Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP (6).

Additional Names : cIAP (CT), cIAP
Description
Left: Western blot analysis of c-IAP in human lung lysate with c-IAP antibody at 1 (lane A), 2 (lane B), and 4 (lane C) µg/ml, respectively.

Below: Immunohistochemistry of cIAP in human lung cells with cIAP antibody at 10 µg/ml.

Other Product Images
Source : c-IAP antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human c-IAP1 c-IAP antibody detects both c-IAP1 and c-IAP2.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : cIAP antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human cIAP (C-Terminus) Peptide (Cat. No. 3325P)

Application : cIAP antibody can be used for the detection of c-IAP by Western blot at 1 to 2 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3325P - cIAP Peptide

Long-Term Storage : cIAP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1331 - Human Lung Tissue Lysate

Species Reactivity :H, M

GI Number : 4502141

Accession Number : NP_001157

Short Description : (CT) An apoptosis inhibitor protein

References
1.Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90.
2.Rothe M, Pan M-G, Henzel WJ, et al. The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins. Cell 1995; 83:1243-52.
3.Deveraux QL, Leo E, Stennicke HR, et al. IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J. 1998; 17:2215-23.
4.Li X, Yang Y, Ashwell JD. TNF-RII and c-IAP1 mediate ubiquitinization and degradation of TRAF 2. Nature 2002; 416:345-7.