Detection in Situ Hybridization

Detection in Situ Hybridization

High throughput microarray transcription analyses provide us with the expression profiles for large amounts of plant genes. However, their tissue and cellular resolution is limited. Thus, for detailed functional analysis, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level.

Hybridization

Hybridization is the process, discovered by Alexander Rich, of combining complementary, single-stranded nucleic acids into a single molecule. Nucleotides will bind to their complement under normal conditions, so two perfectly complementary strands will bind to each other readily. This is called annealing. However, due to the different molecular geometries of the nucleotides, a single inconsistency between the two strands will make binding between them more energetically unfavorable.

Biology & Hybrid

In biology, hybrid has two meanings. The first meaning is the result of interbreeding between two animals or plants of different taxa. Hybrids between different species within the same genus are sometimes known as interspecific hybrids or crosses. Hybrids between different sub-species within a species are known as intra-specific hybrids. Hybrids between different genera are sometimes known as intergeneric hybrids.