RPA Interacting Protein Antibody

RPA Interacting Protein Antibody

Catalog# :4013

Replication protein A (RPA) is a single-stranded-DNA binding protein involved in numerous eukaryotic DNA processes including replication, repair and recombination. RPA interacting protein (RPA IP) has been identified as an adapter protein that is involved in RPA nuclear import instead of the prototypical importin proteins that normally mediate nuclear import. Multiple isoforms of RPA IP are known to exist, with the longest isoform localized to the cytoplasm. Isoform 2 is sumoylated and is located in the PML nuclear body within the nucleus. It has been suggested that this isoform mediates the localization of the RPA complex into the PML nuclear body, thereby participating in RPA function in DNA metabolism.

Additional Names : RPA Interacting Protein (CT), Replication protein A interacting protein, RPAIN, RIP, hRIP, RPA IP

Description
Left: Western blot analysis of RPA Interacting Protein in Jurkat cell lysate with RPA Interacting Protein antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunohistochemistry of RPA Interacting Protein in mouse stomach tissue with RPA Interacting Protein antibody at 2.5 µg/ml.


Source :RPA IP antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human RPA IP.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : RPA Interacting Protein antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : RPA Interacting Protein antibody can be used for detection of RPA Interacting Protein by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4013P - RPA Interacting Protein Peptide
Long-Term Storage : RPA Interacting Protein antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1205 - Jurkat Cell Lysate
2. Cat. No. 1415 - Mouse Stomach Tissue Lysate
Species Reactivity : H, M, R
GI Number : 74136541
Accession Number : NP_001028174
Short Description : (CT) Replication protein A interacting protein
References
1. Zou Y, Liu Y, Wu X, et al. Functions of human replication protein A (RPA): from DNA replication to DNA damage and stress responses. J. Cell Physiol. 2006; 208:267-73.
2. Jullien D, Gorlich D, Laemmli UK, et al. Nuclear import of RPA in Xenopus egg extracts requires a novel protein XRIPalpha but not importin alpha. EMBO J. 1999; 18:4348-58.
3. Park J, Seo T, Kim H, et al. Sumoylation of the novel protein hRIPbeta is involved in replication protein A deposition in PML nuclear bodies. Mol. Cell Biol. 2005; 25:8202-14.(06-01D)