RNAse H2A Antibody

RNAse H2A Antibody
Catalog# :4979
Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.
Additional Names : Rnse H2A, Ribonuclease H2 subunit A, RNAse HI large subunit, Aicadi-Goutieres, syndrome 4 protein, AGS4

DescriptionLeft: Western blot analysis of RNAse H2A in HeLa cell lysate with RNAse H2A antibody at 1 µg/ml.
Below: Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 µg/ml.
Other Product Images

Source :RNAse H2A antibody was raised against a 17 amino acid peptide near the center of human RNAse H2A.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : RNAse H2A antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : RNAse H2A antibody can be used for detection of RNAse H2A by Western blot at 1 – 2 µg/ml.

Tested Application(s) : E, WB, ICCBuffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide :Cat.No. 4979P - RNAse H2A Peptide

Long-Term Storage : RNAse H2A antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control : 1. Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity : H, M, RGI Number : 20981704Accession Number : O75792

Short Description : Ribonuclease H2 subunit AReferences Stein H and Hausen P. Enzyme from calf thymus degrading the RNA moiety of DNA-RNA hybrids: effect on DNA-dependent RNA polymerase. Science 1969; 166:393-5.Cerritelli SM and Crouch RJ.

Ribonuclease H: the enzymes in eukaryotes. FEBS J. 2009; 276:1494-505.Jeong HS, Backlund PS, Chen HC, et al. RNase H2 of Saccharomyces cerevisiae is a complex of three proteins. Nuc. Acids Res. 2004; 32:407-14.Crow YJ, Leitch A, Hayward BE, et al. Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection. Nat. Genet. 2006; 38:910-6.