PRTFDC1 Antibody

PRTFDC1 Antibody

Catalog# :4775

Phosphoribosyl transferase domain containing 1 (PRTFDC1) is highly homologous to the hypoxanthine phosphoribosyltransferase (HPRT1) and may have arisen from a gene duplication event of a common ancestor gene. Recently, it was shown that CpG islands in the PRTFDC1 promoter could be hypermethylated in ovarian cancers and oral squamous-cell carcinomas (OSCC), leading to gene silencing. Restoration of PRTFDC1 expression in OSCC inhibited cell growth in colony-formation assays, while knockdown of PRTFDC1 expression in OSCC that expressed the gene promoted cell growth. These results suggest that PRTFDC1 can act as a tumor-suppressor gene. At least three isoforms of PRTFDC1 are known to exist.

Additional Names : PRTFDC1, Phosphoribosyl transferase domain containing 1, HHGP

Description
Left: Western blot analysis of PRTFDC1 in human brain tissue lysate with PRTFDC1 antibody at 1 µg/ml in the (A) absence and (B) presence of blocking peptide.









Source :PRTFDC1 antibody was raised against a 14 amino acid peptide from near the center of human PRTFDC1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : PRTFDC1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : PRTFDC1 antibody can be used for detection of PRTFDC1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4775P - PRTFDC1 Peptide
Long-Term Storage : PRTFDC1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 9910262
Accession Number : NP_064585
Short Description : Phosphoribosyl transferase domain containing 1
References
1. Nicklas JA. Pseudogenes of the human HPRT1 gene. Environ. Mol. Mutagen 2006; 47:212-8.
2. Keebaugh AC, Sullivan RT, NISC Comparative Sequencing Program, et al. Gene duplication and inactivation in the HPRT gene family. Genomics 2007; 89:134-42.
3. Cai LY, Abe M, Izumi S, et al. Identification of PRTFDC1 silencing and aberrant promoter methylation of GPR150, ITGA8 and HOXD11 in ovarian cancers. Life Sci. 2007; 80:1458-65.
4. Suzuki E, Imoto I, Pimkhaokham A, et al. PRTFDC1, a possible tumor-suppressor gene, is frequently silenced in oral squamous-cell carcinoma by aberrant promoter hypermethylation. Oncogene 2007; 26:7921-32.