DNA Radioactive Oligo Labeling

DNA Radioactive Oligo Labeling

An Allele Specific Oligonucleotide (or ASO) is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay. It is a common tool used in genetic testing, forensics, and Molecular Biology research.

Amino-Modified Oligos

A primary amino function can be incorporated during oligonucleotide synthesis at either the 5'or 3' end or internally using an amino DT reagent. In order to successfully conjugate haptens or fluorophores to the primary amino group, it is necessary to ensure that the oligonucleotide has sodium as the counter-ion rather than ammonium, since the latter may interfere with the conjugation reaction.

Modified Oligos

Modified oligos terminated with the 5'-Thiol-Modifier C6 is shipped with the trityl protecting group still attached to the sulfur. In order to free the thiol group for use removes the trityl group and reduces the sulfur. Oligonucleotides terminated with the 5'-Thiol-Modifier C6 SS (Disulfide) will need to be reduced before use.