SCO2 Antibody

SCO2 Antibody

Catalog# :4045

Synthesis of cytochrome c oxidase 2 was initially identified in yeast as one of two cytochrome c oxidase (COX) assembly proteins that enable the assembly of cytochrome c holoenzyme, a complex that catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. Like their yeast homologs, the function of both SCO2 and SCO1 are dependent on copper ion binding. Recent studies suggest that SCO2 expression is regulated by p53, so that a decrease in p53 expression, such as in numerous tumors and cells lines, the drop in SCO2 expression leads to a shift from normal aerobic respiration towards the production of glycolytic ATP. Defects in the SCO2 protein are also associated with fatal infantile cardioencephalomyopathy and COX deficiency.

Additional Names : SCO2, Synthesis of cytochrome c oxidase 2, cytochrome oxidase deficient homolog 2
Description
Left: Western blot analysis of SCO2 in human liver tissue lysate with SCO2 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.

Below: Immunohistochemistry of SCO2 in human liver tissue with SCO2 antibody at 2.5 µg/ml.

Other Product Images
Source :SCO2 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human SCO2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : SCO2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : SCO2 antibody can be used for detection of SCO2 by Western blot at 0.5 – 1µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4045P - SCO2 Peptide
Long-Term Storage : SCO2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity : H
GI Number : 8134662
Accession Number : O43819
Short Description : Synthesis of cytochrome c oxidase 2
References
1. Glerum DM, Shtanko A, and Tzagoloff A. SCO1 and SCO2 act as high copy suppressors of a mitochondrial copper recruitment defect in Saccharomyces cerevisiae. J. Biol. Chem. 1996; 271:20531-5.
2. Horng Y-C, Leary SC, Cobine PA, et al. Human Sco1 and Sco2 function as copper-binding proteins. J. Biol. Chem. 2005; 280:34113-22.
3. Matoba S, Kang J-G, Patino WD, et al. p53 regulates mitochondrial respiration. Science 2006; 312:1650-3.
4. Papadopoulou LC, Sue CM, Davidson MM, et al. Fatal infantile cardioencephalomyopathy with COX deficiency and mutations in SCO2, a COX assembly gene. Nat. Genetics 1999; 23:333-7 (07-01D)