Catalog# :5167
SH3BP4 was initially isolated as a novel cDNA using differential display analysis using cultured human corneal fibroblasts. The protein contains three Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. SH3BP4 has been shown to interact with specific endocytic proteins such as clathrin, dynamin, and the transferrin receptor (TfR) and localizes to TfR-containing coated pits and vesicles. It is thought to be involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of TfR. At least two isoforms of SH3BP4 are known to exist.
Additional Names : SH3BP4, SH3 domain binding protein 4, TTP, BOG25
DescriptionLeft: Western blot analysis of SH3BP4 in rat lung tissue lysate with SH3BP4 antibody at (A) 1 and (B) 2 µg/ml.
Below: Immunohistochemistry of SH3BP4 in human lung tissue with SH3BP4 antibody at 5 μg/ml.
Other Product Images
Source :SH3BP4 antibody was raised against an 18 amino acid peptide from near the carboxy terminus of human SH3BP4.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : SH3BP4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : SH3BP4 antibody can be used for detection of SH3BP4 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5167P - SH3BP4 Peptide
Long-Term Storage : SH3BP4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1462 - Rat Lung Tissue Lysate
Species Reactivity : H, M, R
GI Number : 7657562
Accession Number : NP_055336
Short Description : SH3 domain binding protein 4
References
1. Dunlevy JR, Berryhill BL, Vergnes JP, et al. Cloning, chromosomal localization, and characterization of cDNA from a novel gene, SH3BP4, expressed by human corneal fibroblasts. Genomics 1999; 62:519-24.
2. Tosoni D, Puri C, Confalonieri S, et al. TTP specifically regulates the internalization of the transferrin receptor. Cell 2005; 123:875-888.
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