2X SYBR Green/Fluorescein qPCR Master Mix

2X SYBR Green/Fluorescein qPCR Master Mix

Catalog# : NB-28111

Price/Unit : $155.00

Unit : 100 reactions

M.W. g/mol

2X SYBR Green/Fluorescein qPCR Master Mix

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)

Features and Benefits | Specifications

BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix is a ready-to-use solution that contains components required for quantitative PCR amplification with Bio-Rad iCycler series of real time PCR systems (iQ®, MyiQTM, iQ5TM). The mix includes Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, a PCR enhancer/stabilizer, SYBR Green and Fluorescein. The user needs only to add template, the primer set and water to the Master Mix to set up the qPCR reaction. This convenient 2X SYBR Green/Fluorescein qPCR Master Mix reduces the time required to set up qPCR reactions and reduces the possibility of contamination particularly when preparing large numbers of reactions. Fluorescein was included in the mix allowing dynamic well factors collection for Bio-Rad iCycler series (iQ®, MyiQTM, iQ5TM) machines. The optimized master mix produces a reliable and sensitive result for specific quantification of target DNA or cDNA from genomic, plasmid and viral templates.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix is available in 3 convenient sizes:
Cat # 28111 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # 28109 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # 28110 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

FEATURES AND BENEFITS

• Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the qPCR reaction.
• Time Savings - Set up qPCR reactions in a shorter time, as less pipetting steps are required.
• Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
• High Sensitivity and yield – The optimized Master Mix allows for highly sensitive amplifications with high yields of qPCR products.
• Robust Amplification - BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.
  

APPLICATIONS AND DATA

• Routine quantitative PCR amplification of DNA templates or cDNA templates
• qPCR for post reverse transcription step

Figure 1. Uniform and Reproducible Results. BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix was used to amplify a 10-fold dilution series (5 ng–500 fg) of Staphylococcus sp. genomic DNA using primers specific to NADPH-1 gene. Duplicate reactions at each concentration were amplified on the iCycler iQ real-time system. From observing the graph above it can be seen that BSI’s Master Mix provided uniform and reproducible results at all the various template concentrations.

SPECIFICATIONS

Reagents supplied – Cat# 28111

2X SYBR Green/Fluorescein qPCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions

Reagents supplied – Cat# 28109

2X SYBR Green/Fluorescein qPCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions

Reagents supplied – Cat# 28110

2X SYBR Green/Fluorescein qPCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Long-Term Storage : Norgen’s 2X SYBR Green/Fluorescein qPCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis

2X PCR Master Mix

2X PCR Master Mix

Catalog# : NB-28009

Price/Unit : $339.00

Unit : 500 reactions

2x PCR Master Mix

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)

Features and Benefits | Specifications

BSI's 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

BSI’s 2X PCR Master Mix is available in 3 convenient sizes:

Cat # NB-28007 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # NB-28008 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # NB-28009 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

FEATURES AND BENEFITS

• Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction.
• Time Savings - Set up PCR reactions in a shorter time, as less pipetting steps are required.
• Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
• High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products.
• Robust Amplification - BSI’s 2X PCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.
  

APPLICATIONS AND DATA

• Routine PCR amplification of DNA templates
• PCR for post reverse transcription step
• Multiple band detection or genotyping

Figure 1. Successful Amplification of Different Sized Amplicons. BSI’s 2X PCR Master Mix was used in a series of PCR reactions to amplify a number of different sized amplicons from the adenovirus E2 region. Lanes 1 and 2 correspond to a 189 bp product, Lanes 3 and 4 correspond to a 565 bp product, Lanes 5 and 6 correspond to a 1501 bp product, and Lanes 7 and 8 correspond to a 2202 bp product. BSI’s 2X PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield. Lane M1 is BSI’s PCR Sizer 100 bp DNA Ladder (Cat #NB-11400) and Lane M2 is BSI’s MidRanger 1kb DNA Ladder (Cat # NB-11700).

SPECIFICATIONS

Figure 1. Highly Stable after Multiple Freeze-Thaw Cycles The stability of BSI’s 2X Master Mix was tested by subjected the mix to multiple freezing and thawing cycles, followed by using the Master Mix to amplify the 16s rDNA (1.5 kb) of Staphylococcus aureus gDNA. BSI’s 2X Master Mix showed robustness and outstanding stability from multiple freezing and thawing steps at -20ºC. M: FastRunner DNA Ladder (Cat#NB-12800). Ten μL of each PCR reaction was loaded on a 1% TAE agarose gel.

Reagents Supplied - Cat # 28007

2X PCR Master Mix (1 Vial, 100 Reactions) Sufficient reagent for 100 x 20 µL reactions

Reagents Supplied – Cat # 28008

2X PCR Master Mix (2 Vials, 100 Reactions each) Sufficient reagent for 200 x 20 µL reactions

Reagents Supplied – Cat # 28009

2X PCR Master Mix (5 Vials, 100 Reactions each) Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
BSI’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Long-Term Storage : BSI’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis

2x PCR Master Mix

2x PCR Master Mix

Catalog# : NB-28008

Price/Unit : $182.20

Unit : 200 reactions

M.W. g/mol

2x PCR Master Mix

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)

Features and Benefits | Specifications

BSI's 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

BSI’s 2X PCR Master Mix is available in 3 convenient sizes:

Cat # NB-28007 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # NB-28008 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # NB-28009 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

FEATURES AND BENEFITS

• Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction.
• Time Savings - Set up PCR reactions in a shorter time, as less pipetting steps are required.
• Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
• High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products.
• Robust Amplification - BSI’s 2X PCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.
  

APPLICATIONS AND DATA

• Routine PCR amplification of DNA templates
• PCR for post reverse transcription step
• Multiple band detection or genotyping

Figure 1. Successful Amplification of Different Sized Amplicons. BSI’s 2X PCR Master Mix was used in a series of PCR reactions to amplify a number of different sized amplicons from the adenovirus E2 region. Lanes 1 and 2 correspond to a 189 bp product, Lanes 3 and 4 correspond to a 565 bp product, Lanes 5 and 6 correspond to a 1501 bp product, and Lanes 7 and 8 correspond to a 2202 bp product. BSI’s 2X PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield. Lane M1 is BSI’s PCR Sizer 100 bp DNA Ladder (Cat #NB-11400) and Lane M2 is BSI’s MidRanger 1kb DNA Ladder (Cat # NB-11700).

SPECIFICATIONS
Figure 1. Highly Stable after Multiple Freeze-Thaw Cycles The stability of BSI’s 2X Master Mix was tested by subjected the mix to multiple freezing and thawing cycles, followed by using the Master Mix to amplify the 16s rDNA (1.5 kb) of Staphylococcus aureus gDNA. BSI’s 2X Master Mix showed robustness and outstanding stability from multiple freezing and thawing steps at -20ºC. M: FastRunner DNA Ladder (Cat#NB-12800). Ten μL of each PCR reaction was loaded on a 1% TAE agarose gel.

Reagents Supplied - Cat # 28007

2X PCR Master Mix (1 Vial, 100 Reactions) Sufficient reagent for 100 x 20 µL reactions

Reagents Supplied – Cat # 28008

2X PCR Master Mix (2 Vials, 100 Reactions each) Sufficient reagent for 200 x 20 µL reactions

Reagents Supplied – Cat # 28009

2X PCR Master Mix (5 Vials, 100 Reactions each) Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
BSI’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Long-Term Storage : BSI’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis

2X PCR Master Mix

2X PCR Master Mix

Catalog# : NB-28007

Price/Unit : $117.80

Unit : 100 reactions

2x PCR Master Mix

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)

Features and Benefits | Specifications

BSI's 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

BSI’s 2X PCR Master Mix is available in 3 convenient sizes:

Cat # NB-28007 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # NB-28008 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # NB-28009 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

FEATURES AND BENEFITS

• Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction.
• Time Savings - Set up PCR reactions in a shorter time, as less pipetting steps are required.
• Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
• High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products.
• Robust Amplification - BSI’s 2X PCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.
  

APPLICATIONS AND DATA

• Routine PCR amplification of DNA templates
• PCR for post reverse transcription step
• Multiple band detection or genotyping

Figure 1. Successful Amplification of Different Sized Amplicons. BSI’s 2X PCR Master Mix was used in a series of PCR reactions to amplify a number of different sized amplicons from the adenovirus E2 region. Lanes 1 and 2 correspond to a 189 bp product, Lanes 3 and 4 correspond to a 565 bp product, Lanes 5 and 6 correspond to a 1501 bp product, and Lanes 7 and 8 correspond to a 2202 bp product. BSI’s 2X PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield. Lane M1 is BSI’s PCR Sizer 100 bp DNA Ladder (Cat #NB-11400) and Lane M2 is BSI’s MidRanger 1kb DNA Ladder (Cat # NB-11700).

SPECIFICATIONS
Figure 1. Highly Stable after Multiple Freeze-Thaw Cycles The stability of BSI’s 2X Master Mix was tested by subjected the mix to multiple freezing and thawing cycles, followed by using the Master Mix to amplify the 16s rDNA (1.5 kb) of Staphylococcus aureus gDNA. BSI’s 2X Master Mix showed robustness and outstanding stability from multiple freezing and thawing steps at -20ºC. M: FastRunner DNA Ladder (Cat#NB-12800). Ten μL of each PCR reaction was loaded on a 1% TAE agarose gel.

Reagents Supplied - Cat # 28007

2X PCR Master Mix (1 Vial, 100 Reactions) Sufficient reagent for 100 x 20 µL reactions

Reagents Supplied – Cat # 28008

2X PCR Master Mix (2 Vials, 100 Reactions each) Sufficient reagent for 200 x 20 µL reactions

Reagents Supplied – Cat # 28009

2X PCR Master Mix (5 Vials, 100 Reactions each) Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
BSI’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Long-Term Storage : BSI’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis

2X One-Step RT-PCR Master Mix

2X One-Step RT-PCR Master Mix

Catalog# : NB-28115

Price/Unit : $1,270.00

Unit : 500 reactions

2x One-Step RT-PCR Master Mix

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)

Features and Benefits | Specifications

BSI’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA temples with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with BSI’s 2X One-Setp RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.

BSI’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:

Cat # NB-28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # NB-28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # NB-28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

FEATURES AND BENEFITS

• Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the RT-PCR reaction.
• Time Savings - Set up RT-PCR reactions in a shorter time, as less pipetting steps are required.
• Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
• High Sensitivity and yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products.
• Robust Amplification - BSI’s 2X One-Step RT-PCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.
  

APPLICATIONS AND DATA
Figure 1. Successful Amplification of Different Sized Amplicons. BSI’s 2X One-Step RT-PCR Master Mix was used in RT-PCR reactions to amplify a number of different amplicons. Lanes 1 and 2 correspond to the E. coli 16s rRNA gene (large amplicon), which is 1.5 kb in size. Lanes 3 and 4 correspond to the E. coli 16s rRNA gene (small amplicon), which is 550 bp in size. Lanes 5 and 6 correspond to the human S15 gene, which is 360 bp in size. Lanes 7 and 8 correspond to the human 5S rRNA gene, which is 120 bp in size. BSI’s 2X One-Step RT-PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield.

SPECIFICATIONS
Figure 2. Robust Amplification from Different Amounts of RNA Input. BSI’s 2X One Step RT-PCR Master Mix was used to amplify the human S15 transcripts from a dilution series of HeLa total RNA isolated using BSI’s Total RNA Purification Kit. The 361 bp amplicon was robustly amplified from a broad range of RNA inputs, from the ng range down to fg range.

Reagents supplied – Cat # NB-28113

2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions

Reagents supplied – Cat # NB-28114

2X One-Step RT-PCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions

Reagents supplied – Cat # NB-28115

2X One-Step RT-PCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Long-Term Storage : 2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis

2X One-Step RT-PCR Master Mix

2X One-Step RT-PCR Master Mix

Catalog# : NB-28114

Price/Unit : $605.00

Unit : 200 reactions

M.W. g/mol

2x One-Step RT-PCR Master Mix

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)

Features and Benefits | Specifications BSI’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA temples with high yields of PCR products. Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with BSI’s 2X One-Setp RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV. BSI’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes: Cat # NB-28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume) Cat # NB-28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume) Cat # NB-28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume) FEATURES AND BENEFITS

• Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the RT-PCR reaction.
• Time Savings - Set up RT-PCR reactions in a shorter time, as less pipetting steps are required.
• Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
• High Sensitivity and yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products.
• Robust Amplification - BSI’s 2X One-Step RT-PCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.
  

APPLICATIONS AND DATA
Figure 1. Successful Amplification of Different Sized Amplicons. BSI’s 2X One-Step RT-PCR Master Mix was used in RT-PCR reactions to amplify a number of different amplicons. Lanes 1 and 2 correspond to the E. coli 16s rRNA gene (large amplicon), which is 1.5 kb in size. Lanes 3 and 4 correspond to the E. coli 16s rRNA gene (small amplicon), which is 550 bp in size. Lanes 5 and 6 correspond to the human S15 gene, which is 360 bp in size. Lanes 7 and 8 correspond to the human 5S rRNA gene, which is 120 bp in size. BSI’s 2X One-Step RT-PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield.

SPECIFICATIONS
Figure 2. Robust Amplification from Different Amounts of RNA Input. BSI’s 2X One Step RT-PCR Master Mix was used to amplify the human S15 transcripts from a dilution series of HeLa total RNA isolated using BSI’s Total RNA Purification Kit. The 361 bp amplicon was robustly amplified from a broad range of RNA inputs, from the ng range down to fg range.

Reagents supplied – Cat # NB-28113

2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions

Reagents supplied – Cat # NB-28114

2X One-Step RT-PCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions

Reagents supplied – Cat # NB-28115

2X One-Step RT-PCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Long-Term Storage : X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis

2X One-Step RT-PCR Master Mix

2X One-Step RT-PCR Master Mix

Catalog# : NB-28113

Price/Unit : $360.00

Unit : 100 reactions

2x One-Step RT-PCR Master Mix

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)

Features and Benefits | Specifications

BSI’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA temples with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with BSI’s 2X One-Setp RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.

BSI’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:

Cat # NB-28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # NB-28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # NB-28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

FEATURES AND BENEFITS

• Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the RT-PCR reaction.
• Time Savings - Set up RT-PCR reactions in a shorter time, as less pipetting steps are required.
• Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
• High Sensitivity and yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products.
• Robust Amplification - BSI’s 2X One-Step RT-PCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.
  

APPLICATIONS AND DATA

Figure 1. Successful Amplification of Different Sized Amplicons. BSI’s 2X One-Step RT-PCR Master Mix was used in RT-PCR reactions to amplify a number of different amplicons. Lanes 1 and 2 correspond to the E. coli 16s rRNA gene (large amplicon), which is 1.5 kb in size. Lanes 3 and 4 correspond to the E. coli 16s rRNA gene (small amplicon), which is 550 bp in size. Lanes 5 and 6 correspond to the human S15 gene, which is 360 bp in size. Lanes 7 and 8 correspond to the human 5S rRNA gene, which is 120 bp in size. BSI’s 2X One-Step RT-PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield.

SPECIFICATIONS
Figure 2. Robust Amplification from Different Amounts of RNA Input. BSI’s 2X One Step RT-PCR Master Mix was used to amplify the human S15 transcripts from a dilution series of HeLa total RNA isolated using BSI’s Total RNA Purification Kit. The 361 bp amplicon was robustly amplified from a broad range of RNA inputs, from the ng range down to fg range.

Reagents supplied – Cat # NB-28113

2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions

Reagents supplied – Cat # NB-28114

2X One-Step RT-PCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions

Reagents supplied – Cat # NB-28115

2X One-Step RT-PCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Long-Term Storage : 2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis

dNTP Set

dNTP Set

Catalog# : NB-28104

Price/Unit : $241.00

Unit : 4 x 0.25 ml

M.W. g/mol

BSI’s dNTP Set (Cat# NB-28104) contains one tube each of dATP, dCTP, dGTP and dTTP at a concentration of 100 mM, supplied in water.

BSI’s dNTP Mixes contain dATP, dCTP, dGTP and dTTP at a concentration of either 10 mM each (Cat # NB-28106) or 25 mM each (Cat # NB-28105) supplied in water.

BSI’s highly pure dNTP Set and dNTP Mixes can be used in all standard and sensitive PCR applications.

Applications

• Standard PCR
• Multiplex PCR
• Genotyping
• Methylation-Sensitive PCR (MSP)
• Nested PCR
• One-Step RT-PCR
• Two-Step RT-PCR
• Multiplex RT-PCR

Quality Assurance
dNTP solutions are certified free of nucleases and phosphatases.

Long-Term Storage : BSI's dNTP Set and dNTP Mixes should be stored at –20°C. Avoid repeated freeze-thawing.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis

dNTP Mix (25 mM)

dNTP Mix (25 mM)

Catalog# : NB-28105

Price/Unit : $297.00

Unit : 1 ml

BSI’s dNTP Set (Cat# NB-28104) contains one tube each of dATP, dCTP, dGTP and dTTP at a concentration of 100 mM, supplied in water.

BSI’s dNTP Mixes contain dATP, dCTP, dGTP and dTTP at a concentration of either 10 mM each (Cat # NB-28106) or 25 mM each (Cat # NB-28105) supplied in water.

BSI’s highly pure dNTP Set and dNTP Mixes can be used in all standard and sensitive PCR applications.

Applications

• Standard PCR
• Multiplex PCR
• Genotyping
• Methylation-Sensitive PCR (MSP)
• Nested PCR
• One-Step RT-PCR
• Two-Step RT-PCR
• Multiplex RT-PCR
  

Quality Assurance
dNTP solutions are certified free of nucleases and phosphatases.
Long-Term Storage : dNTP Set and dNTP Mixes should be stored at –20°C. Avoid repeated freeze-thawing.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis

dNTP Mix (10 mM)

dNTP Mix (10 mM)

Catalog# : NB-28106

Price/Unit : $178.00

Unit : 1 ml

BSI’s dNTP Set (Cat# NB-28104) contains one tube each of dATP, dCTP, dGTP and dTTP at a concentration of 100 mM, supplied in water.

BSI’s dNTP Mixes contain dATP, dCTP, dGTP and dTTP at a concentration of either 10 mM each (Cat # NB-28106) or 25 mM each (Cat # NB-28105) supplied in water.

BSI’s highly pure dNTP Set and dNTP Mixes can be used in all standard and sensitive PCR applications.

Applications

• Standard PCR
• Multiplex PCR
• Genotyping
• Methylation-Sensitive PCR (MSP)
• Nested PCR
• One-Step RT-PCR
• Two-Step RT-PCR
• Multiplex RT-PCR
  

Quality Assurance
dNTP solutions are certified free of nucleases and phosphatases.

Long-Term Storage : dNTP Set and dNTP Mixes should be stored at –20°C. Avoid repeated freeze-thawing.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis

CAD Antibody

CAD Antibody

Catalog# : 2007

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Additional Names : CAD (I17), DFF40 (I17)

Description
Left: Western blot analysis of CAD in mouse kidney tissue lysate with CAD antibody at 2 µg/ml. Below: Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 2 µg/ml.

Source :CAD antibody was raised against a peptide corresponding to 17 amino acids near the center of murine CAD.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : CAD antibody was raised in rabbit.

Immunogen : Human CAD (I17) Peptide (Cat. No. 2007P)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2007P - CAD Peptide

Long-Term Storage : CAD antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1405 - Mouse Kidney Tissue Lysate
  

Species Reactivity :M, R

GI Number : 20137695

Accession Number : O54788

Short Description : (I17) Caspase Activated DNase

References

1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
3. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
4. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40

For more detail regarding Peptide please visit here : Custom Peptide Synthesis

CAD Antibody

CAD Antibody

Catalog# : 2011

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei (1-4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Additional Names : CAD (CT), DFF40
Description
Left: Western blot analysis of CAD in mouse lung (L) and kidney (K) tissue lysates with CAD antibody at 1:500 dilution. Below: Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 1 µg/ml.

Source :CAD antibody was raised against a peptide corresponding to amino acids 314 to 329 of murine CAD.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : CAD antibody was raised in rabbit.

Immunogen : Human CAD (C-Terminus) Peptide (Cat. No. 2011P)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2011P - CAD Peptide

Long-Term Storage : CAD antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1402 - Mouse Lung Tissue Lysate
• Cat. No. 1405 - Mouse Kidney Tissue Lysate
  

Species Reactivity :M

GI Number : 20137695

Accession Number : O54788

Short Description : (CT) Caspase Activated DNase

References

1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
3. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
4. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40

For more detail regarding Peptide please visit here : Custom Peptide Synthesis

Bnip3L Antibody

Bnip3L Antibody

Catalog# : 2289

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3alpha, and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3alpha/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2,3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

Additional Names : Bnip3L, Nix
Description
Left: Western blot analysis of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide (2289P) with Bnip3L antibody (IN) at 1 µg/ml. Below: Immunohistochemical staining of human kidney tissue using Bnip3L antibody at 2 µg/ml.

Source :Bnip3L antibody was raised against a peptide corresponding to amino acids 77 to 92 of human origin, which are identical to those of mouse Bnip3L.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bnip3L antibody was raised in rabbit.

Immunogen : Human Bnip3L (Intermediate Domain) Peptide (Cat. No. 2289P)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2289P - Bnip3L Peptide

Long-Term Storage : Bnip3L antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1204 - K562 Cell Lysate
• Cat. No. 1305 - Human Kidney Tissue Lysate
  

Species Reactivity :H

GI Number : 4138825

Accession Number : AF067396

Short Description : A new member in Bcl2 family

References

1. Matsushima M, Fujiwara T, Takahashi E, et al. Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998;21:230-5
2. Yasuda M, Han JW, Dionne CA, Boyd JM, Chinnadurai G. BNIP3alpha: a human homolog of mitochondrial proapoptotic protein BNIP3. Cancer Res 1999;59:533-7
3. Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J Biol Chem 1999;274(1):7-10
4. Imazu T, Shimizu S, Tagami S, et al. Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability. Oncogene. 1999;18:4523-9 (RD1200)

For more detail regarding Peptide please visit here : Custom Peptide Synthesis

Bmf Antibody

Bmf Antibody

Catalog# : 3031

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor) (1). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2). Bmf is constitutively expressed in many tissues (1,2).

Additional Names : Bmf (NT), Bmf
Description
Left: Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with Bmf antibody at 2 µg /ml. Below: Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 µg/ml.

Source :EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bmf antibody was raised in rabbit.

Immunogen : Human Bmf (N-Terminus) Peptide (Cat. No. 3031P)

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3031P - Bmf Peptide

Long-Term Storage : Bmf antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1211 - HepG2 Cell Lysate
• Cat. No. 1210 - 293 Whole Cell Lysate
• Cat. No. 1201 - HeLa Cell Lysate
  

Species Reactivity :H, M

GI Number : 15723378

Accession Number : NP_277038

Short Description : (NT) A new member in Bcl-2 family

References

1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L, Cheney RE, Huang DC, Strasser A. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science. 2001;293(5536):1829-32.
2. Hunt A, Evan G. Apoptosis. Till death us do part. Science. 2001;293(5536):1784-5.

Bmf Antibody

Bmf Antibody

Catalog# : 3029

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor) (1). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2). Bmf is constitutively expressed in many tissues (1,2).

Additional Names : Bmf (CT), Bmf

Description
Below: Immunofluorescence of Bmf in human kidney tissue with Bmf antibody at 10 µg/ml.

Source :Bmf antibody was raised with a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human Bmf.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bmf antibody was raised in rabbit.

Immunogen : Human Bmf (C-Terminus) Peptide (Cat. No. 3029P)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3029P - Bmf Peptide

Long-Term Storage : Bmf antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1305 - Human Kidney Tissue Lysate
  

Species Reactivity :H

GI Number : 15723378

Accession Number : NP_277038

Short Description : (CT) A new member in Bcl-2 family

References

1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L, Cheney RE, Huang DC, Strasser A. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science. 2001;293(5536):1829-32.
2. Hunt A, Evan G. Apoptosis. Till death us do part. Science. 2001;293(5536):1784-5.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis

Bit1 Antibody

Bit1 Antibody

Catalog# : 3605

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2).

Additional Names : Bit1 (IN), Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2

Description
Left: Western blot analysis of Bit1 in Daudi cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 µg/ml. Below: Immunohistochemistry of Bit1 in human small intestine tissue with Bit1 antibody at 10 µg/ml.

Source :Bit1 antibody was raised against a 16 amino acid peptide from near the center of human Bit1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bit1 antibody was raised in rabbit.

Immunogen : Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (Intermediate Domain)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3605P - Bit1 Peptide

Long-Term Storage : Bit1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1224 - Daudi Cell Lysate
• Cat. No. 1308 - Human Small Intestine Tissue Lysate
  

Species Reactivity :H, M

GI Number : 7706351

Accession Number : NP_057161

Short Description : (IN) Bcl-2 inhibitor of transcription 1

References

1. Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
2. Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.
3. Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis

Bit1 Antibody

Bit1 Antibody

Catalog# : 3603

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2)

Additional Names : Bit1 (NT), Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2
Description
Left: Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 µg/ml. Below: Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 µg/ml.

Source : Bit1 antibody was raised against a 15 amino acid peptide from near the amino-terminus of human Bit1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bit1 antibody was raised in rabbit.

Immunogen : Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (N-Terminus) Peptide

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3603P - Bit1 Peptide

Long-Term Storage : Bit1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1288 - A-20 Cell Lysate
• Cat. No. 1212 - 3T3 Whole Cell Lysate
  

Species Reactivity :H, M, R

GI Number : 7706351

Accession Number : NP_057161

Short Description : (NT) Bcl-2 inhibitor of transcription 1

References

1. Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis

Bim Monoclonal Antibody

Bim Monoclonal Antibody

Catalog# : PM-4821

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

Additional Names : Bim (1C2H4): Bcl-2 interacting protein, Bcl-2-related ovarian death gene, BOD, Bcl-2-like 11
Description
Left: Western blot analysis of 5 ng of Bim recombinant protein with Bim antibody at 1 µg/ml. Below: Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 µg/ml.






Source :Mouse monoclonal Bim antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim.

Purification : Immunoaffinity chromotography purified IgG

Clonality and Clone : This is a monoclonal antibody. (Clone 1C2H4)

Host : Bim monoclonal antibody was raised in mouse.

Immunogen : Mouse monoclonal Bim antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Long-Term Storage : Bim monoclonal antibody can be stored at 4ºC, stable for one year.

Positive Control

• Cat. No. 95-107 - Bim Recombinant Protein
  

Species Reactivity :H, M, R

Short Description : A Bcl-2 interacting protein

References

• O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
• Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis