DNA RNA Hybrid

DNA RNA Hybrid

The helix is unwound, the coding strand consists of unpaired bases, whilst the template strand consists of an RNA:DNA hybrid, followed by a number of unpaired bases at the rear. This hybrid consists of the most-recently-added nucleotides of the RNA transcript, complementary base-paired to the template strand. The number of base-pairs in the hybrid is under investigation, but it has been suggested that the hybrid is formed from the last 10 nucleotides added. Bio-Synthesis provides custom DNA RNA hybrid synthesis with various base, ribose, and backbone modifications.

One-Hybrid

The one-hybrid variation of this technique is designed to investigate protein-DNA interactions and uses a single fusion protein in which the AD is linked directly to the binding domain. The binding domain in this case however is not necessarily of fixed sequence as in two-hybrid protein-protein analysis but may be constituted by a library. This library can be selected against the desired target sequence which is inserted in the promoter region of the reporter gene construct. In a positive-selection system, a binding domain which successfully binds the UAS and allows transcription will thus be selected.

Two-hybrid Screening

Two-hybrid screening or 2-hybrid screening is a molecular biology technique used to discover protein-protein interactions and protein-DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.

Three-Hybrid

RNA-protein interactions have been investigated through a three-hybrid variation of the two-hybrid technique. In this case, a hybrid RNA molecule serves to adjoin together the two protein fusion domains which aren't intended to interact with each other but rather the intermediary RNA molecule.