Catalog# :5071
Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2,289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling. This antibody is predicted to not cross-react with PCDH18.
Additional Names : PCDH12, Protocadherin 12, vascular endothelial cadherin 2, VE-cadherin 2, VECAD2
DescriptionLeft: Western blot analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 2 µg/ml.
Below: Immunohistochemistry of PCDH12 in rat lung tissue with PCDH12 antibody at 5 µg/ml.
Other Product Images
Source :
PCDH12 antibody was raised against a 17 amino acid peptide near the amino terminus of human PCDH12.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : PCDH12 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : PCDH12 antibody can be used for detection of PCDH12 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5071P - PCDH12 Peptide
Long-Term Storage : PCDH12 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1204 - K562 Cell Lysate
Species Reactivity : H, M, R
GI Number : 7706113
Accession Number : NP_057664
Short Description : Protocadherin 12
References
1. Frank M and Kemler R. Protocadherins. Curr. Opin. Cell Biol. 2002; 14:557-62.
2. Telo P, Breviaro F, Huber P, et al. Identification of a novel cadherin (vascular endothelial cadherin-2) located at intercellular junctions in endothelial cells. J. Biol. Chem. 1998; 28:17565-72.
3. Rampon C, Prandini MH, Bouillot S, et al. Protocadherin 12 (VE-cadherin 2) is expressed in endothelial, trophoblast, and mesangial cells. Exp. Cell Res. 2005; 302:48-60.
4. Rampon C, Bouillot S, Climescu-Haulica A, et al. Protocadherin 12 deficiency alters morphogenesis and transcriptional profile of the placenta. Physiol. Genomics 2008; 34:193-204.
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