Bio-Synthesis provide LNA oligonucleotides probes by using state-of-the art automatic synthesis platform to give fully modified LNA or spiked oligonucleotide synthesis which LNA-modified DNA, LNA-modified RNA or, for example, LNA-modified 2’-O-Me-RNA oligonucleotides with PTOs/PS mix linkages. The fact that LNA monomers readily can be mixed with a variety of natural or synthetic nucleic acid monomers and labels results in great design flexibility.
Oligonucleotides
Oligonucleotides are chemically synthesized using nucleotides, called phosphoramidites, normal nucleotides which have protection groups: preventing amine, hydroxyl groups and phosphate groups interacting incorrectly. One phosphoramidite is added at the time, the product's 5' phosphate is deprotected and a new base is added and so on (backwards), at the end, all the protection groups are removed. Nevertheless, being a chemical process, several incorrect interactions occur leading to some defective products.
Locked Nucleic Acid
A locked nucleic acid (LNA), often referred to as inaccessible RNA, is a modified RNA nucleotide. The ribose moiety of an LNA nucleotide is modified with an extra bridge connecting the 2' and 4' carbons. The bridge "locks" the ribose in the 3'-endo structural conformation, which is often found in the A-form of DNA or RNA. LNA nucleotides can be mixed with DNA or RNA bases in the oligonucleotide whenever desired.
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