Catalog# :2265
Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. Despite its predicted molecular weight, Nephrin often migrates at a lower than expected size in SDS-PAGE.
Additional Names : Nephrin, NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor
DescriptionLeft: Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 µg/ml in the (A) absence and (B) presence of blocking peptide.
Below: Immunohistochemistry of Nephrin in mouse kidney tissue with Nephrin antibody at 1 µg/ml.
Other Product Images
Source : Nephrin antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Nephrin.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Nephrin antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : Nephrin antibody can be used for detection of Nephrin by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 2265P - Nephrin Peptide
Long-Term Storage : Nephrin antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1405 - Mouse Kidney Tissue Lysate
Species Reactivity : H, M, R
GI Number : 4758822
Accession Number : NP_004637
Short Description : Kidney glomerulus-specific cell adhesion receptor
References
1. Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein – Nephrin – is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.
2. Tryggvason K. Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm. J. Am. Soc. Nephrol. 1999; 10:2440-5.
3. Tryggvason K and Wartiovaara J. Molecular basis of glomerular permselectivity. Curr, Opin. Nephrol. Hypertens. 2001; 10:543-9. (07-01D)
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