Catalog# :4707
The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-160 is part of the hetero-oligomeric complex that also contains NUP107, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP160 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP160 may be an important drug target in HIV treatment. Multiple isoforms of NUP160 are known to exist.
Additional Names : NUP160 (CT), Nucleoporin 160, nuclear pore complex 160
DescriptionLeft: Western blot analysis of NUP160 in rat brain tissue lysate with NUP160 antibody at (A) 0.5 and (B) 1 µg/ml.
Below: Immunohistochemistry of NUP160 in human brain tissue with NUP160 antibody at 2.5 μg/ml.
Other Product Images
Source :NUP160 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human NUP160.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : NUP160 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : NUP160 antibody can be used for detection of NUP160 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4707P - NUP160 Peptide
Long-Term Storage : NUP160 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1203 - A549 Cell Lysate
Species Reactivity : H, M, R
GI Number : 119588287
Accession Number : EAW67881
Short Description : (CT) Nucleoporin 160
References
1. Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell 2006; 125:1041-53.
2. Boehmer T, Enninga J, Dales S, et al. Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc. Natl. Acad. Sci. USA 2003; 100:981-5.
3. Orjalo AV, Arnaoutov A, Shen Z, et al. The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol. Bio. Cell 2006; 17:3806-18.
4. Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.
1 comment:
Hello Friends,
The nuclear pore complex is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. NUP160 has recently been identified as an HIV dependency factor, that may be an important drug target in HIV treatment. Thanks a lot!
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