NUP155 Antibody

NUP155 Antibody

Catalog# :4703

The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. One protein in this assembly is the nucleoporin (NUP)-155, which is localized symmetrically to both the nucleoplasmic and cytoplasmic faces of the NPC. NUP155 has been reported to interact with both NUP35 and the mRNA export factor Gle1, but the functions of these interactions are still unknown. NUP155 has also been shown to be required for NPC assembly and nuclear envelope (NE) membrane fusion. NUP155 is recruited late in NE formation, suggesting that NUP155 defines an essential late step in NE assembly. NUP155 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP155 may be an important drug target in HIV treatment. At least two isoforms of NUP155 are known to exist.

Additional Names : NUP155 (CT), Nucleoporin 155, nuclear pore complex 155

nuclear pore complex 155

Description
Left: Western blot analysis of NUP155 in P815 cell lysate with NUP155 antibody at (A) 0.5 and (B) 1 µg/ml.





Source : NUP155 antibody was raised against a 18 amino acid peptide from near the carboxy terminus of human NUP155.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : NUP155 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : NUP155 antibody can be used for detection of NUP155 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4703P - NUP155 Peptide
Long-Term Storage : NUP155 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1286 - P815 Cell Lysate
Species Reactivity : H, M
GI Number : 24430149
Accession Number : NP_705618
Short Description : (CT) Nucleoporin 155
References
1. Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell 2006; 125:1041-53.
2. Radu A, Blobel G and Wozniak RW. Nup155 is a novel nuclear pore complex protein that contains neither repetitive sequence motifs nor reacts with WGA. J. Cell Biol. 1993; 121:1-9.
3. Hawryluk-Gara LA, Shibuya EK, and Wozniak RW. Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex. Mol. Biol. Cell 2005; 16:2382-94.
4. Rayala HJ, Kendirgi F, Barry DM, et al. The mRNA export factor Gle1 interacts with the nuclear pore complex protein Nup155. Mol. Cell Proteomics 2004; 3:145-55.