2X One-Step RT-PCR Master Mix

2X One-Step RT-PCR Master Mix Catalog# : NB-28114 Price/Unit : $605.00 Unit : 200 reactions M.W. g/mol 2x One-Step RT-PCR Master Mix

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)

Features and Benefits | Specifications BSI’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA temples with high yields of PCR products. Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with BSI’s 2X One-Setp RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV. BSI’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes: Cat # NB-28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume) Cat # NB-28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume) Cat # NB-28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume) FEATURES AND BENEFITS

• Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the RT-PCR reaction.
• Time Savings - Set up RT-PCR reactions in a shorter time, as less pipetting steps are required.
• Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
• High Sensitivity and yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products.
• Robust Amplification - BSI’s 2X One-Step RT-PCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.

APPLICATIONS AND DATA Figure 1. Successful Amplification of Different Sized Amplicons. BSI’s 2X One-Step RT-PCR Master Mix was used in RT-PCR reactions to amplify a number of different amplicons. Lanes 1 and 2 correspond to the E. coli 16s rRNA gene (large amplicon), which is 1.5 kb in size. Lanes 3 and 4 correspond to the E. coli 16s rRNA gene (small amplicon), which is 550 bp in size. Lanes 5 and 6 correspond to the human S15 gene, which is 360 bp in size. Lanes 7 and 8 correspond to the human 5S rRNA gene, which is 120 bp in size. BSI’s 2X One-Step RT-PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield. SPECIFICATIONS Figure 2. Robust Amplification from Different Amounts of RNA Input. BSI’s 2X One Step RT-PCR Master Mix was used to amplify the human S15 transcripts from a dilution series of HeLa total RNA isolated using BSI’s Total RNA Purification Kit. The 361 bp amplicon was robustly amplified from a broad range of RNA inputs, from the ng range down to fg range. Reagents supplied – Cat # NB-28113 2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions Reagents supplied – Cat # NB-28114 2X One-Step RT-PCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions Reagents supplied – Cat # NB-28115 2X One-Step RT-PCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions Storage Conditions and Product Stability 2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year. Long-Term Storage : X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year. Product Usage For laboratory research use only, not for diagnostic use! Not for use in humans.

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