AIF Antibody

AIF Antibody

Catalog# : 2301

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (2).

Additional Names : AIF (CT), AIF

Description
Left: Western blot analysis of AIF in K562 with AIF antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml. Below: Immunocytochemistry of AIF in K562 cells with AIF antibody at 5 µg/ml.



Source : AIF antibody was raised against a peptide corresponding to amino acids 593 to 606 of human AIF. This sequence is identical to those of mouse and rat AIF.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : AIF antibody was raised in rabbit.

Immunogen : Human AIF (C-Terminus) Peptide (Cat. No. 2301P)

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2301P - AIF Peptide

Long-Term Storage : AIF antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1204 - K562 Cell Lysate
• Cat. No. 1404 - Mouse Liver Tissue Lysate
• Cat. No. 1464 - Rat Liver Tissue Lysate

Species Reactivity :H, M, R

GI Number : 50400606

Accession Number : O95381

Short Description : (CT) Apoptosis Inducing Factor

References

1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8
2. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6 (WD0800)

For more detail regarding Peptide please visit here : Custom Peptide Synthesis