Catalog# : NB-28087
Price/Unit : $213.00
Unit : 1000 units (5 U/ul)
Taq Polyermase
Recombinant source
Functionality preserved at wide range of temperatures
Highly reliable and reproducible reactions
Supplied with detergent-free, Mg-free Reaction Buffer for compatibility with microarray and DHPL applications and reactions that require adjustment of magnesium concentrations
Compatible with the use of modified nucleotides (dUTP, dITP, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
Available in 2 different concentrations: 5 units/µL and 1 unit/µL
Features and Benefits | Specifications
Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The enzyme is supplied with a detergent-free, Mg-free Reaction Buffer, making it compatible with microarray and DHPLC applications and reactions that require adjustment of magnesium concentration.
BSI's Taq DNA Polymerase with 10X Taq Reaction Buffer (Mg-Free) is available in 2 convenient sizes:
- 1000 units at a concentration of 5000 units/mL (Cat # NB-28087)
- 200 units at a concentration of 1000 unitls/mL (Cat# NB-28093)
Enzyme Source:
An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1
APPLICATIONS
- Routine PCR
- Preparation of PCR products for cloning
- Primer extension
- Microarray analysis
- DHPLC
- DNA labelling
- DNA sequencing
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SPECIFICATIONS
Reagents supplied - Cat# 28087:
1000 units of Taq DNA Polymerase (5000 units/mL)
4 x 1.25 mL of 10X Taq Reaction Buffer (Mg-Free)
1.25 mL of 25 mM MgCl2
Reagents supplied - Cat# 28093
200 units of Taq DNA Polymerase (1000 units/mL)
1.25 mL of 10X Taq Reaction Buffer (Mg-Free)
1.25 mL of 25 mM MgCl2
Reaction Conditions:
1X Taq Reaction Buffer (Mg-Free) - (10 mM Tris-HCl, 50 mM KCl, pH 8.3 @ 25°C)
Taq Storage Buffer:
50 mM Tris-HCl pH 8 at 25°C, 50 mM KCl, 50mM NaCl, 0.5 mM Dithiothreitol, 0.1 mM EDTA, 50% Glycerol, 1% Triton X-100
Storage Temperature:
-20°C
Long-Term Storage : Store in -20°C.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.
For more detail regarding Peptides please visit here : Custom Peptide Synthesis
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