AATF Antibody

AATF Antibody

Catalog# : 3039

AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK) (1), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases (2). AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways (3). Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the b-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer’s disease (AD) (4,5), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.

Additional Names : AATF, Apoptosis antagonizing transcription factor, Che-1

Description
Left: Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Below: Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 µg/ml.

Source : AATF antibody was raised against a 12 amino acid peptide from near the carboxy terminus of human AATF.

Purification : Immunoaffinity purified IgG

Clonality and Clone : This is a polyclonal antibody.

Host : AATF antibody was raised in rabbit.

Immunogen : Human AATF Peptide (Cat. No. 3039P)

Application : AATF antibody can be used for

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3039P - AATF Peptide

Long-Term Storage : AATF antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1308 - Human Small Intestine Tissue Lysate

Species Reactivity : H, M

GI Number : 7657013

Accession Number : NP_036270

Short Description : An anti-apoptotic transcription factor

References

1. AATF antibody can be stored at 4ºC, stable for one year. Page G, Lodige I, Kogel D et al. AATF, a novel transcription factor that interacts with Dlk/ZIP kinase and interferes with apoptosis. FEBS Lett. 1999; 462:187-191.
2. Kawai T, Matsumoto M, Takeda K, et al. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol. Cell Biol. 1998; 18:1642-51.
3. Lindfors K, Halttunen T, Huotari P, et al. Identification of novel transcription factor-like gene from human intestinal cells. Biochem. Biophys. Res. Commun. 2000; 276:660-6.
4. Xie J and Guo Q. AATF protects neural cells against oxidative damage induced by amyloid b-peptide. Neurobiol. of Dis. 2004; 16:150-7.

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