Milk Bacterial DNA Isolation

Milk Bacterial DNA Isolation

Catalog# : NB-21500

Price/Unit : $196.00

Unit : 25 preps

This Milk Bacterial DNA Isolation Kit provides a rapid method for the isolation and purification of genomic DNA from the various bacterial species found within milk. The kit allows for the isolation of genomic DNA from both Gram negative and Gram positive bacteria in milk samples.

Purification is based on spin column chromatography using Biosynthesis proprietary resin as the separation matrix. The genomic DNA is preferentially purified from other cellular proteinaceous components. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR, quantitative PCR and Southern Blot analysis.

Features

• Genomic DNA can be detected from as little as 10 bacterial cells in 1 mL of milk
• Isolate genomic DNA from both Gram negative and Gram positive bacteria in milk
• Fast and Efficient

Milk Bacterial DNA Isolation Kit Benefits

• Isolate genomic DNA from all types of bacteria found in milk - Bacterial genomic DNA can be isolated from both Gram negative and Gram positive bacteria using the kit.
• Fast and easy processing- Rapid spin-column format allows for the processing of 10 samples in 45 minutes.
• Isolate high quality DNA - No degradation of the genomic DNA isolated with the Milk Bacterial Genomic DNA Isolation Kit is observed.
• Isolate genomic DNA from milk samples with very low bacterial densities - Genomic DNA can be isolated and detected from milk samples with bacterial concentrations as low as 10 bacterial cells in 1 mL of milk.
• Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA can be used in a number of downstream applications including restriction enzyme digestions, PCR amplifications, quantitative PCR and Southern Blot analysis.

Applications

• Restriction Enzyme Digestions
• PCR
• Quantitative PCR
• Southern Blot Analysis
  

Figure 1. Isolation and Detection of DNA from as Little as 10 Bacterial Cells in 1 mL of Milk

Increasing amounts of bacteria (10^1 , 10^3 , 10^5 , 10^7 ) were added to pasteurized milk samples, and the bacterial DNA was subsequently isolated using this Milk Bacterial DNA Isolation Kit. The isolated genomic DNA was then detected using real-time PCR. Bacterial genomic DNA could be isolated and detected from all the 1 mL milk samples, including the sample that contained only 10 bacterial cells (blue line).
Figure 2. End-Point PCR of Milk Bacterial DNA samples

1 mL of milk was spiked with various amount of E coli DH5a. Bacterial DNA was isolated in duplicate using this Milk Bacterial DNA Isolation Kit and 1 uL of each elution was used for conventional PCR. 10 uL of each PCR was loaded on an agarose gel. The amount of bacteria in each milk sample is as follows: A = 10^7, B = 10^5, C = 10^3, D = 10^1. Lane X is a negative control (no template) and Lane M is Biosynthesis PCRSizer 100bp DNA Ladder.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

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