BAC DNA MiniPrep

BAC DNA MiniPrep

Catalog# : NB-18000

Price/Unit : $110.00

Unit : 25 preps

The BAC DNA MiniPrep Kit is designed for the rapid preparation of BACs (bacterial artificial chromosomes) and other large DNA constructs from small batch cultures of Escherichia coli. Purification is based on spin column chromatography using Biosynthesis proprietary resin as the separation matrix. This resin binds DNA under high salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The process does not require ethanol or other alcohols, a significant improvement over other techniques to achieve full compatibility with alcohol sensitive applications.

The purified DNA is free of genomic DNA, RNA and cellular proteins and is suitable for many downstream applications. The BAC DNA MiniPrep Kit typically recovers between 0.6 and 1µg of DNA from 3.0 mL of bacterial culture. Purification of constructs up to 130 kb in size have been verified.
Features

• High Recovery: 0.6 to 1 µg of BAC DNA can be purified from 3.0 mL of bacterial culture.
• Alcohol-free protocol: No alcohols are used durig the purification process, thus the DNA is compatible with alcohol sensitive downstream applications.
• Fast and Easy Processing: Rapid spin-column format allows for the processing of multiple samples in 1 hour.
• Recovered DNA is suitable for downstream applications: Purified BAC DNA is fully compatible with restriction enzyme digestions and sequencing.
• High Binding Capacity of Columns: The binding capacity of the columns in the BAC DNA MiniPrep Kit is 25 µg

Figure 1. Isolation of BAC DNA Using Biosynthesis BAC DNA MiniPrep Kit and a Competitor Kit. BAC DNA was isolated in triplicate from 3 mL overnight cultures. 20% of recovered DNA was loaded in each lane. Lanes 1-3 are the Biosynthesis BAC DNA MiniPrep Kit, Lanes 4-6 are Competitor X and M is the Biosynthesis UltraRanger 1kb DNA Ladder (#12100).

Figure 2. Full Compatibility with Restriction Digests. BAC DNA isolated with the BAC DNA MiniPrep Kit is fully digestible. In Figure 2, two different isolated BACs were digested with Hind III (Lanes 3 and 4). Lanes 1 and 2 are uncut plasmid, and Lane M is the Biosynthesis UltraRanger 1kb DNA ladder (#12100).

For more detail regarding Peptide please visit here : Custom Peptide Synthesis