BAD Antibody

BAD Antibody

Catalog# : 3343

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death (reviewed in 1 and 2). Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax (3). Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival (4), whereas JNK-induced phosphorylation promotes the apoptotic role of Bad (5).

Additional Names : BAD, Bcl-2-like 8

Description
Left: Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml. Below: Immunohistochemical staining of rat thymus using Bad at 2 µg/ml.


Source : Bad antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : BAD antibody was raised in rabbit.

Immunogen : Murine Bad / Bcl-2-like 8 Peptide (Cat. No. 3343P)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3343P - Bad Peptide

Long-Term Storage : BAD antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1213 - T24 Cell Lysate
2. Cat. No. 1471 - Rat Thymus Tissue Lysate
   

Species Reactivity :H, M, R

GI Number : 17371773

Accession Number : Q92934

Short Description : Bcl-2 family member

References

1. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
2. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
3. Ottilie S, Diaz JL, Horne W, et al. Dimerization properties of human BAD. Identification of a BH-3 domain and analysis of its binding to mutant BCL-2 and BCL-XL proteins. J. Biol. Chem. 1997; 272:30866-72.
4. Zhou XM, Liu Y, Payne G, et al. Growth factors inactivate the cell death promoter BAD by phosphorylation of its BH3 domain on Ser155. J. Biol. Chem. 2000; 275:25046-51.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis