AES Antibody

AES Antibody

Catalog# : 3609

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).

Additional Names : AES (NT), Amino-terminal enhancer of split / GRG / ESP1 / TLE5
Description

Left: Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 µg/ml. Below: Immunocytochemistry of AES in 293 cells with AES antibody at 10 µg/ml.

Source : AES antibody was raised against a 16 amino acid peptide from near the amino-terminus of human AES.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : AES antibody was raised in rabbit.

Immunogen : Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (N-Terminus) Peptide (Cat. No. 3609)

Application : AES antibody can be used

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3609P - AES Peptide

Long-Term Storage : AES antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1210 - 293 Cell Lysate

Species Reactivity : H, M

GI Number : 39812019

Accession Number : NP_945320

Short Description : (NT) Amino-terminal enhancer of split

References

1. Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
2. Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52.
3. Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.
4. Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis