ARF-BP1 Antibody

ARF-BP1 Antibody

Catalog# : 4213

The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.

Additional Names : ARF-BP1, ARF binding protein 1; HECT, UBA and WWE domain containing 1; HUWE1; Mcl-1 ubiquitin ligase E3; Mule

Description
Left: Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 µg/ml। Below: Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 µg/ml.



Source : ARF-BP1 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1।

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : ARF-BP1 antibody was raised in rabbit.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 4213P - ARF-BP1 Peptide

Long-Term Storage : ARF-BP1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1224 - Daudi Cell Lysate
  

Species Reactivity :H, M, R

GI Number : 61676188

Accession Number : NP_113584

Short Description : ARF binding protein

References

1. Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol. 2006; 38:1637-41.
2. Pomerantz J, Schreiber-Agus N, Leigeois NJ, et al. The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2’s inhibition of p53. Cell 1998; 92:725-34.
3. Chen D, Kon N, Li M, et al. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell 2005; 121:1071-83.
4. Zhong Q, Gao W, Du F, et al. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell 2005; 121:1085-95.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis