Catalog# : 4659
CLIP170 was initially identified as a new type of intermediate filament associated protein that is highly expressed in Reed-Sternberg cells, the tumoral cells diagnostic for Hodgkin’s disease. Later experiments showed that it is located at microtubule plus ends and is required for the binding of endocytic carrier vesicles. CLIP170 has also been suggested to act with LIS1, a protein implicated in brain development, to regulate dynein/dynactin binding microtubules. Other studies suggest that CLIP170 can influence the formation of lamellipodia and cell invasion by invasive breast cancer cells by regulating the release of kinesin and IQGAP1 from a complex of those proteins, CLIP170 and Rac1. At least two isoforms of CLIP170 are known to exist.
Additional Names : CLIP170, CAP-Gly domain-containing linker protein 1, CLIP, CYLN1, Restin, RSN
DescriptionLeft: Western blot analysis of CLIP170 in rat brain tissue lysate with CLIP170 antibody at (A) 0.5 and (B) 1 µg/ml.
Below: Immunofuorescence of CLIP170 in human brain tissue with CLIP170 antibody at 20 μg/ml.
Other Product Images
Source : CLIP170 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human CLIP170.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CLIP170 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : CLIP170 antibody can be used for detection of CLIP170 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4659P - CLIP170 Peptide
Long-Term Storage : CLIP170 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity :H, M, R
GI Number : 4506751
Accession Number : NP_002947
Short Description : CAP-Gly domain-containing linker protein 1
References
1. Bilbe G, Delabie J, Bruggen J, et al. Restin: a novel intermediate filament-associated protein highly expressed in the Reed-Sternberg cells of Hodgkin’s disease. EMBO J. 1992; 11:2103-13.
2. Diamantopoulos GS, Perez F, Goodson HV, et al. Dynamic localization of CLIP-170 to microtubule plus ends is coupled to microtubule assembly. J. Cell Biol. 1999; 144:99-112.
3. Pierre P, Scheel J, Richard JE, et al. CLIP-170 links endocytic vesicles to microtubules. Cell 70:887-900.
4. Suzuki K and Takahashi K. Regulation of lamellipodia formation and cell invasion by CLIP-170 in invasive human breast cancer cells. Biochem. Biophys. Res. Commun. 2008; 368:199-204.
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