TNFRSF14 Antibody

TNFRSF14 Antibody

Catalog# : 3773

Tumor necrosis factor receptor (TNFR) superfamily members are defined by cysteine-rich domains in their extracellular regions that bind TNF-related ligands that share a common structural homology in their extracellular domain. TNFRSF14 was initially identified as the Herpesvirus entry mediator and upon binding to the herpes simplex virus (HSV) envelope glycoprotein D or either of its natural ligands LIGHT and lymphotoxin alpha (LT), activates the transcription factors NF-kappaB and AP-1. Activation of this signal transduction pathway in T cells stimulates T cell proliferation and cytokine production, leading to inflammation and enhanced CTL-mediated tumor immunity, suggesting that these proteins may be useful as potential targets for controlling cellular immune responses.
Additional Names : TNFRSF14 (CT), Tumor necrosis factor receptor superfamily 14, Herpesvirusentry mediator, HVEM, TR2
Description
Left: Western blot analysis of TNFRSF14 in Raji cell lysate with TNFRSF14 antibody at 2 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Below: Immunocytochemistry of TNFRSF14 in Raji with TNFRSF14 antibody at 10 µg/ml.

Other Product Images
Source : TNFRSF14 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human TNFRSF14.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : TNFRSF14 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : TNFRSF14 antibody can be used for the detection of TNFRSF14 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 3773P - TNFRSF14 Peptide
Long-Term Storage : TNFRSF14 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1207 - Raji Cell Lysate
Species Reactivity :H
GI Number : 13878821
Accession Number : Q92956
Short Description : (CT) Herpesvirus entry mediator
References
1. Watts TH. TNF/TNFR family members in costimulation of T cell responses. Annu. Rev. Immunol. 2005; 23:23-68.
2. Montgomery RI, Warner MS, Lum BJ, et al. Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family. Cell 1996; 87:427-36.
3. Marsters SA, Ayres TM, Skubatch M, et al. Herpesvirus entry mediator, a member of the tumor necrosis factor receptor (TNFR) family, interacts with members of the TNFR-associated factor family and activates the transcription factors NF-kappaB and AP-1. J. Biol. Chem. 1997; 272:14029-32.
4. Mauri DN, Ebner R, Montgomery RI, et al. LIGHT, a new member of the TNF superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator. Immunity 1998; 8:21-30.