Catalog# : 3777
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism (reviewed in 1). The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging (2). beta actin is one of six different actin isoforms which have been identified. Like GAPDH, Β-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Additional Names : Beta-actin (CT), Beta actin
DescriptionLeft: Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.
Below: Immunocytochemistry of beta-actin in HeLa cells with beta-actin antibody at 10µg/ml.
Other Product Images
Source : b-actin antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human b-actin.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Beta-actin antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human b-actin / Beta actin (C-Terminus) Peptide (Cat. No. 3777P)
Application : b-actin antibody can be used for the detection of b-actin by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3777P - B-actin Peptide
Long-Term Storage : Beta-actin antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity :H, M, R
GI Number : 12803203
Accession Number : AAH02409
Short Description : (CT) a major cytoskeletal protein, C Terminus
References
1. Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
2. Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.
1 comment:
Hello all,
Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes.Alpha actin expression is limited to various types of muscle, whereas beta and gamma actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. Thanks a lot!
Beta-actin antibody
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