Friday, October 1, 2010

CTRP2 Antibody

CTRP2 Antibody

Catalog# : 3561

Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-?-related proteins (CTRPs) 1-7 (2). Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues (3). These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). Of the CTRPs, CTRP2 is most similar structurally and functionally to adiponectin. Recombinant CTRP2 rapidly activated AMPK and MAPK in cultured C2C12 cells, leading to increased glycogen accumulation and fatty acid oxidation (3).

Additional Names : CTRP2 (NT), CTRP2

Left: Western blot analysis of CTRP2 in Caco-2 cell lysate with CTRP2 antibody at 1 µg/ml in either the (A) absence or (B) presence of blocking peptide.

Source : CTRP2 antibody was raised against a 16 amino acid peptide from near the amino-terminus of human CTRP2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CTRP2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Murine CTRP2 (N-Terminus) Peptide (Cat. No. 3561P)
Application : CTRP2 antibody can be used for the detection of CTRP2 by Western blot at 1 µg/ml.Caco-2 cell lysate can be used as positive control. Anti-CTRP2 is human and mouse reactive. These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3561P - CTRP2 Peptide
Long-Term Storage : CTRP2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1223 - Caco-2 Cell Lysate
Species Reactivity :H, M
GI Number : 94818738
Accession Number : NP_114114
Short Description : (NT) a novel adipokine

1. Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
2. Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
3. Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
4. Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.

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