CSN8 Antibody

CSN8 Antibody

Catalog# : 4601

The COP9 signalosome (CSN) is an evolutionarily conserved protein complex of the eight subunits that interacts with deubiquitinating enzymes and protein kinases and is highly homologous to the lid sub-complex of 26S proteasome. The CSN complex is an essential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the SCF-type E3 ligase complexes, which leads to a decrease in ubiquitin ligase activity of SCF-comlpexes such as SCF, CSA or DDB2. It is also involved in phosphorylation of p53, c-jun/JUN, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN8 encodes the smallest and the least conserved but first identified subunit of CSN. Recent studies show CSN8 is essential for Drosophila development and is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence.
Additional Names : CSN8 (CT), COP9 signalosome complex subunit 8, SGN8, JAB1-containing signalosome subunit 8
Description
Left: Western blot analysis of CSN8 in Human liver lysate with CSN8 antibody at 2 µg/ml.





Source : CSN8 antibody was raised against a 15 amino acid peptide near the carboxy terminus of the human CSN8.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CSN8 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : CSN8 antibody can be used for detection of CSN8 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4601P - CSN8 Peptide
Long-Term Storage : CSN8 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity :H, M, R
GI Number : 5729779
Accession Number : NP_006701
Short Description : (CT) COP9 signalosome complex subunit 8
References

1. Wei N and Deng XW. The COP9 signalosome. Annu. Rev. Cell Dev. Biol. 2003; 19:261-86.
2. Bech-Otschir D, Seeger M, and Dubiel W. The COP9 signalosome: at the interface between signal transduction and ubiquitin-dependent proteolysis. J. Cell Sci. 2002; 115:467-73.
3. Groisman R, Polanowska J, Kuraoka I, et al. The ubiquitin ligase activity in the DDB2 and regulated by the COP9 signalosome in response to DNA damage. Cell 2003; 113:357-67.
4. Uhle S, Medalia O, Waldron R, et al. Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome. EMBO J. 2003; 22:1302-12.