DNA MRNA

DNA MRNA

Bio-Synthesis continued to provide quality mRNA products for your gene knockdown studies. At Bio-Synthesis we offer disovery scale synthesis to mid-scale multi gram quantities of antisense oligos for clinical diagnostic applications, in addition to the synthesis of these modified oligos, we routinely assist customers in the design of the oligos that are particularly suited to their applications.

MRNA Display

MRNA display is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. The process results in translated peptides or proteins that are associated with their mRNA progenitor via a puromycin linkage. The complex then binds to an immobilized target in a selection step (affinity chromatography). The mRNA-protein fusions that bind well are then reverse transcribed to cDNA and their sequence amplified via a polymerase chain reaction. The end result is a nucleotide sequence that encodes a peptide with high affinity for the molecule of interest.

Complementary DNA

In genetics, complementary DNA (cDNA) is DNA synthesized from a mature mRNA template in a reaction catalyzed by the enzyme reverse transcriptase.[ cDNA is often used to clone eukaryotic genes in prokaryotes. CDNA is also produced by retrovirus (such as HIV-1, HIV-2, Simian Immunodeficiency Virus, etc.) which is integrated into its host to create a provirus.