LSD1 Antibody

LSD1 Antibody

Catalog# :3761

Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.

Additional Names : LSD1 (NT), Lysine-specific histone demethylase, amine oxidase flavin containing domain protein 2, AOF2

Description
Left: Western blot analysis of LSD1 in P815 cell lysate with LSD1 antibody at (A) 1 and (B) 2 µg/ml.




Source :
LSD1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human LSD1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : LSD1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen : Human LSD1 (N-Terminus) Peptide (Cat. No. 3761P)
Application : LSD1 antibody can be used for the detection of LSD1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat. No. 3761P - LSD1 Peptide
Long-Term Storage : LSD1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1286 - P815 Cell Lysate
Species Reactivity : H, M, R
GI Number : 58761544
Accession Number : NP_001009999
Short Description : (NT) A histone demethylase
References
1. Shi Y, Lan F, Matson C, et al. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 2004; 119:941-53.
2. Kouzarides T. Histone methylation in transcriptional control. Curr. Opin. Genet. Dev. 2002; 12:198-209.
3. Shi YJ, Matson C, Lan F, et al. Regulation of LSD1 histone demethylase activity by its associated factors. Mol. Cell 2005; 19:857-64.
4. Nakamura T, Mori T, Tada S, et al. ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation. Mol. Cell 2002; 10:1119-28.