Catalog# :4941
The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. Like the highly homologous LASS5, LASS6 is also an endoplasmic reticulum, multi-pass membrane protein. LASS6 is also involved in the synthesis of C14, C16 and C18-ceramide, but shows a preference for unsaturated fatty acids. LASS6 is broadly expressed in a wide range of tissues and microarray data suggests that it may play a role in cancer differentiation and early embryonic development. At least two isoforms of LASS6 are known to exist. This antibody is predicted not to cross-react with LASS5.
DescriptionLeft: Western blot analysis of LASS6 in rat brain tissue lysate with LASS6 antibody at (A) 1 and (B) 2 µg/ml.
Below: Immunohistochemistry of LASS6 in mouse brain tissue with LASS6 antibody at 2.5 µg/ml.
Other Product Images
Source :LASS6 antibody was raised against a 13 amino acid peptide near the amino terminus of the human LASS6.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : LASS6 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : LASS6 antibody can be used for detection of LASS6 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4941P - LASS6 Peptide
Long-Term Storage : LASS6 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 80478334
Accession Number : AAI09285
Short Description : longetivity, sphingolipids, ceramide synthesis
References
1. Riebeling C, Allegood JC, Wang E, et al. Two mammalian longevity assurance gene (LAG1) family members, Trh1 and Trh, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. J. Biol. Chem. 2003; 278:43452-9.
2. Mizutani Y, Kihara A and Igarashi Y. Mammalian Lass6 and its related family members regulate synthesis of specific ceramides. Biochem. J. 2005; 390:263-71.
3. Lahiri S and Futerman AH. LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor. J. Biol Chem. 2005; 280:33735-8.
4. Weinmann A, Galle PR, and Teufel A. LASS6, an additional member of the longevity assurance gene family. Int. J. Mol. Med. 2005; 16:905-10.
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