GNPDA1 Antibody

GNPDA1 Antibody

Catalog# : 5049

Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate deaminases that are thought to have arisen though gene duplication, with the GNPDA2 protein closer in structure and activity to the E. coli enzyme. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2. GNPDA1 is predicted to not cross-react with GNPDA2.
Additional Names : GNPDA1, Glucosamine-6-phosphate deaminase 1, GNPI, GNPI1
Description
Left: Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunocytochemistry of GNPDA1 in 293 cells with GNPDA1 antibody at 5 μg/ml.

Other Product Images
Source : GNPDA1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human GNPDA1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : GNPDA1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 5049P - GNPDA1 Peptide
Long-Term Storage : GNPDA1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1405 - Mouse Kidney Tissue Lysate
Species Reactivity :H, M, R
GI Number : 2935438
Accession Number : AAC05123
Short Description : Glucosamine-6-phosphate deaminase 1
References

1. Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J. 1998; 12:91-9.
2. Zhang J, Zhang W, Zou D, et al. Cloning and functional characterization of GNPI, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. J. Cell Biochem. 2003; 88:932-40.
3. Arreola R, Valderrama B, Morante ML, et al. Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study. FEBS Lett. 2003; 551:63-70.