GPVI Antibody

GPVI Antibody

Catalog# : 4769

Glycoprotein VI (GP6) is a 58kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. It is uniquely expressed by cells of the megakaryocytic/platelet lineage, and is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRγ) and natural killer receptors. Glycoprotein VI plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcRγ, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. GPVI deficiency can result in bleeding disorders. Further study should reveal the extent of GPVI involvement in thrombotic disease and allow the development of alternative anti-thrombotic compounds.
Additional Names : GPVI (CT), Glycoprotein VI, GPIV, Platelet glycoprotein VI precursor
Description
Left: Western blot analysis of GPVI in A20 lysate with GPVI antibody at 1µg/m in either the absence or (B) the presence of blocking peptide.

Below: Immunohistochemistry of GPVI in human heart tissue with GPVI antibody at 10 μg/ml.

Other Product Images

Source : GPVI antibody was raised against a 15 amino acid peptide near the carboxy terminus of the human GPVI.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : GPVI antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : GPVI antibody can be used for detection of GPVI by Western blot at 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4769P - GPVI Peptide
Long-Term Storage : GPVI antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1288 - A20 Cell Lysate
Species Reactivity :H, M, R
GI Number : 143770755
Accession Number : NP_057447
Short Description : (CT) Platelet glycoprotein VI precursor
References
1. Jarvis GE, Atkinson BT, Snell DC, et al. Distinct roles of GPVI and integrin alpha(2) beta(1) in platelet shape change and aggregation induced by different collagens. Br. J. Pharmacol. 2002; 137:107-17.
2. Inoue O, Suzuki-Inoue K, Dean WL, et al. Integrin alpha2beta1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLCgamma2. J. Cell Biol. 2003;160:769-80.
3. Clemetson JM, Polgar J, Magnenat E, et al. The platelet collagen receptor glycoprotein VI is a member of the immunoglobulin superfamily closely related to FcαR and the natural killer receptors. J Biol. Chem. 1999; 274:29019-24.
4. Jandrot-Perrus M, Busfield S, Lagrue AH, et al. Cloning, characterization, and functional studies of human and mouse glycoprotein VI: a platelet-specific collagen receptor from the immunoglobulin superfamily. Blood 2000; 96:1798-807.