Catalog# : 4973
The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.
Additional Names : Gle1 (IN), RNA export mediator
DescriptionLeft: Western blot analysis of Gle1 in 293 cell lysate with Gle1 antibody at (A) 1 and (B) 2 µg/ml.
Below: Immunohistochemistry of Gle1 in mouse brain tissue with Gle1 antibody at 2.5 µg/ml.
Other Product Images
Source : Gle1 antibody was raised against a 14 amino acid peptide near the center of human Gle1.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Gle1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : Gle1 antibody can be used for detection of Gle1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4973P - Gle1 Peptide
Long-Term Storage : Gle1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1210 - 293 Cell Lysate
Species Reactivity :H, M, R
GI Number : 51317384
Accession Number : NP_001003722
Short Description : (IN) RNA export mediator
References
1. Murphy R and Wente SR. An RNA-export mediator with an essential nuclear export signal. Nature 1996; 383:357-60.
2. Watkins JL, Murphy R, Emtage JL, et al. The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export. Proc. Natl. Acad. Sci. USA 1998; 95:6779-84.
3. Kendirgi F, Rexer DJ, Alcazar-Roman AR, et al. Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: A conserved mechanism in the export of Hsp70 mRNA. Mol. Biol. Cell 2005; 16:4304-15.
4. Rayala HJ, Kendirgi F, Barry DM, et al. The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155. Mol. Cell. Proteomics 2004; 3:145-55.
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