DNA Oligo Synthesis

DNA Oligo Synthesis

BSI has more than 20 years experience in oligonucleotide chemistry. We are not only synthesizing standard DNA/RNA oligonucleotides but are also specialized in production and purification of modified oligomers. Base, ribose and backbone modifications and reporter groups like dyes and haptens are routinely incorporated in DNA, RNA, LNA, and BNA. Through innovative production process and synthesis know-how, our services are the best in their class, providing you with innovative and trustworthy solutions to accelerate your research.

Oligonucleotide synthesis

Typically, synthesized oligonucleotides are single-stranded DNA molecules around 15-20 bases in length. They are most commonly used as primers for DNA sequencing and amplification, as probes for detecting complementary DNA or RNA via molecular hybridization, and for the targeted introduction of mutations and restriction sites, allowing for the synthesis of artificial genes.

Synthesis substrates

Oligonucleotides are chemically synthesized using phosphoramidites. A phosphoramidite is a normal nucleotide with protection groups added to its reactive amine, hydroxyl and phosphate groups. These protection groups prevent unwanted side reactions and force the formation of the desired product during synthesis.

Read here: DNA Oligo Synthesis