GSTP1 Antibody

GSTP1 Antibody

Catalog# : 4413

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism (i.e., the metabolism of environmental mutagens and carcinogens) and may play a role in susceptibility to cancer. More recent experiments have suggested that differential expression of GSTP1 also contributes to the sensitivity of xenobiotics in the substantia nigra and may influence the pathogenesis of reactive oxygen species-induced neurological disorders such as Parkinson’s disease. CpG island hypermethylation of the GSTP1 promoter leading to the silencing of the GSTP1 gene has also been linked to cancer.
Additional Names : GSTP1, Glutathione S-transferase pi

[Image]Description
Left: Western blot analysis of GSTP1 in Jurkat cell lysate with GSTP1 antibody at (A) 0.5 and (B) 1 µg/ml.

Below: Immunohistochemical staining of human lung tissue using GSTP1 antibody at 2.5 µg/ml.

Other Product Images
[Image]Source : GSTP1 antibody was raised against a 14 amino acid peptide from near the center of human GSTP1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : GSTP1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : GSTP1 antibody can be used for the detection of ATG10 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4413P - GSTP1 Peptide
Long-Term Storage : GSTP1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity :H, M, R
GI Number : 4504183
Accession Number : NP_000843
Short Description : Glutathione S-transferase pi
References

1. Pearson WR. Phylogenies of glutathione transferase families. Methods Enzymol. 2005; 401:186-204.
2. Clapper ML. Genetic polymorphism and cancer risk. Curr. Oncol. Rep. 2000; 2:251-6.
3. Smeyne M, Boyd J, Shepherd KR, etc. GSTpi expression mediates dopaminergic neuron sensitivity in experimental parkinsonism. Proc. Natl. Acad. Sci. USA 2007; 104:1977-82.
4. Ellinger J, Bastian PJ, Jurgan T, et al. CpG island hypermethylation at multiple gene sites in diagnosis and prognosis of prostate cancer. Urology 2008; 71:161-7.