FNIP2 Antibody

FNIP2 Antibody

Catalog# : 5057

FNIP2 is the second protein found to interact with folliculin, the product of the Birt-Hogg-Dube (BHD) gene. Folliculin is thought to act as a tumor suppressor as mutations or loss of heterozygosity in this gene are associated with BHD syndrome-related renal tumors. Folliculin and FNIP1, a protein that shares 49% identity to FNIP2, bind to AMPK, an important energy sensor in cells that negatively regulates the mammalian target of rapamycin (mTOR), a protein that is thought to be the master switch for cell growth and proliferation. FNIP1 and FNIP2 are able to form homo- and heteromeric multimers, suggesting these proteins may have a functional relationship. Multiple isoforms of FNIP2 are known to exist. This antibody is predicted to not cross-react with FNIP1.
Additional Names : FNIP2 (NT), Folliculin interacting protein 2, FNIPL


Description
Left: Western blot analysis of FNIP2 in mouse skeletal muscle lysate with FNIP2 antibody at (A) 1 and (B) 2 µg/ml.




Source : FNIP2 antibody was raised against an 18 amino acid peptide near the amino terminus of human FNIP2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : FNIP2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : FNIP2 antibody can be used for detection of FNIP2 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 5057P - FNIP2 Peptide
Long-Term Storage : FNIP2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate
Species Reactivity :H, M, R
GI Number : 154689769
Accession Number : NP_065891
Short Description : (NT) Folliculin interacting protein 2
References
1. Hasumi H, Baba M, Hong S-B, et al. Identification and characterization of a novel folliculin-interacting protein FNIP2. Gene 2008; 415:60-7.
2. Takagi Y, Kobayashi T, Shiono M, et al. Interaction of folliculin (Birt-Hogg-Dube gene product) with novel Fnip1-like (FnipL/Fnip2) protein. Oncogene 2008; 27:5339-47.
3. Vocke CD, Yang Y, Pavlovich CP, et al. High frequency of somatic frameshift BHD mutations in Birt-Hogg-Dube-associated renal tumors. J. Natl. Cancer Inst. 2005; 97:931-5.
4. Baba M, Hong SB, Sharma M, et al. Folliculin encoded by the BHD gene interacts with a binding protein, FNIP1, and AMPK, and is involved in AMPK and mTOR signaling. Proc. Natl. Acad. Sci. USA 2006; 103:15552-7.