GPR15 Antibody

GPR15 Antibody

Catalog# : 2101

Human immunodeficiency virus (HIV) and related viruses require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. The genes encoding human and monkey GPR15/BOB (for G protein-coupled receptor 15 and brother of Bonzo, respectively,) were recently cloned. This novel G protein-coupled receptor serves as corecptor for simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1. The ligand for GPR15 has not been identified yet.
Additional Names : GPR15 (NT), BOB
Description
Left: Western blot analysis of GPR15 in human spleen lysate with GPR15 antibody at (A) 0.5 and (B) 1 µg/ml.





Source : GPR15 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human GPR15. The sequence differs from those of African green monkey and pig-tailed macaque BOB by one amino acid (2).
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : GPR15 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human GPR15 (N-Terminus) Peptide (Cat. No. 2101P)
Application : GPR15 antibody can be used for detection of GPR15/BOB by Western blot at 1:500 to 1:2000 dilution. An approximately 50 kDa band can be detected.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2101P - GPR15 Peptide
Long-Term Storage : GPR15 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1205 - Jurkat Cell Lysate
2. Cat. No. 1203 - A549 Whole Cell Lysate
Species Reactivity :H, M, R
GI Number : 1171145
Accession Number : U34806
Short Description : (NT) HIV coreceptor
References
1. Heiber M, Marchese A, Nguyen T, Heng HH, George SR, O'Dowd BF. A novel human gene encoding a G-protein-coupled receptor (GPR15) is located on chromosome 3. Genomics 1996;32:462-5
2. Deng HK, Unutmaz D, KewalRamani VN, Littman DR. Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 199;388:296-300
3. Farzan M, Choe H, Martin K, Marcon L, Hofmann W, Karlsson G, Sun Y, Barrett P, Marchand N, Sullivan N, Gerard N, Gerard C, Sodroski J. Two orphan seven-transmembrane segment receptors which are expressed in CD4-positive cells support simian immunodeficiency virus infection. J Exp Med 1997;186:405-11 (RD1299)