DcR2 Antibody

DcR2 Antibody

Catalog# : 2021

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL, and DcR1/TRID is a decoy receptor (1-3). Another member of the TRAIL receptor family was more recently identified and designated DcR2, TRAIL-R4, or TRUNDD (4-6). DcR2 has an extracellular TRAIL-binding domain but lacks intracellular death domain and does not induce apoptosis. Like DR4 and DR5, DcR2 transcript is widely expressed in normal human tissues. Overexpression of DcR2 attenuated TRAIL-induced apoptosis.

Additional Names : DcR2 (ID), TRAIL-R4

Description
Left: Western blot analysis of DcR2 in HeLa whole cell lysate with DcR2 antibody at 1:1000 dilution.

Below: Immunocytochemistry staining of HeLa cells using DcR2 antibody at 10µg/ml.

Other Product Images

Source : DcR2 antibody was raised against a peptide corresponding to amino acids 249 to 263 of human DcR2 precursor.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : DcR2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human DcR2 (Intracellular Domain) Peptide (Cat. No. 2021P)

Application : DcR2 antibody can be used for detection of DcR2 expression by Western blot at 1:1000 to 1:2000 dilution.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2021P - DcR2 Peptide

Long-Term Storage : DcR2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity :H, M, R

GI Number : 18203495

Accession Number : Q9UBN6

Short Description : (ID) Decoy Receptor 2 for TRAIL

References
1.Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
2.Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8
3.Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21
4.Marsters SA, Sheridan JP, Pitti RM, Huang A, Skubatch M, Baldwin D, Yuan J, Gurney A, Goddard AD, Godowski P, Ashkenazi A. A novel receptor for Apo2L/TRAIL contains a truncated death domain. Curr Biol 1997;7:1003-6